Strategies for enhancing microbial production of 2′-fucosyllactose, the most abundant human milk oligosaccharide

Y Liu, Y Zhu, H Wang, L Wan, W Zhang… - Journal of Agricultural …, 2022 - ACS Publications
Human milk oligosaccharides (HMOs), a group of structurally diverse unconjugated glycans
in breast milk, act as important prebiotics and have plenty of unique health effects for …

High-Level De Novo Biosynthesis of 2′-Fucosyllactose by Metabolically Engineered Escherichia coli

Y Liu, Y Zhu, L Wan, R Chen, W Zhang… - Journal of Agricultural …, 2022 - ACS Publications
2′-Fucosyllactose (2′-FL) is the most abundant oligosaccharide in human milk. In this
study, a highly efficient biosynthetic route for 2′-FL production was designed via the de …

Engineering Escherichia coli MG1655 for Highly Efficient Biosynthesis of 2′-Fucosyllactose by De Novo GDP-Fucose Pathway

R Chen, Y Zhu, H Wang, Y Liu, J Meng… - Journal of Agricultural …, 2023 - ACS Publications
2′-Fucosyllactose (2′-FL), the most typical human milk oligosaccharide, is used as an
additive in premium infant formula. Herein, we constructed two highly effective 2′-FL …

Rcs signal transduction system in Escherichia coli: composition, related functions, regulatory mechanism, and applications

Z Li, Y Zhu, W Zhang, W Mu - Microbiological Research, 2024 - Elsevier
The regulator of capsule synthesis (Rcs) system, an atypical two-component system
prevalent in numerous gram-negative bacteria, serves as a sophisticated regulatory …

De novo biosynthesis of 2′-fucosyllactose in engineered Pichia pastoris

D Qian, C Zhang, C Deng, M Zhou, L Fan, L Zhao - Biotechnology Letters, 2023 - Springer
Purpose Pichia pastoris is well known for its ability to produce short and low-immunogenic
humanized glycosyl chains onto recombinant glycoproteins, it was thus speculated to be …

[HTML][HTML] Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica

Y Zhang, X Zhang, H Liu, J Hou, M Liu, Q Qi - Synthetic and Systems …, 2023 - Elsevier
Fucosyllactose (2′-FL) has great application value as a nutritional component and the
whole cell biosynthesis of 2′-FL has become the focus of current research. Yarrowia …

Integrated bioprocessing and genetic strategies to enhance soluble expression of anti-HER2 immunotoxin in E. Coli

S Mani, B Arab, V Akbari, CP Chou - AMB Express, 2024 - Springer
Immunotoxins are widely applied for cancer therapy. However, bacterial expression of
immunotoxins usually leads to the formation of insoluble and non-functional recombinant …

A coupled SWATPlus and BiLSTM tuning model for improved daily scale hydroclimate simulation in typical loess hilly areas of China

X Zhang, J Liu, H Ren, Y Yang, J Zhu - Natural Hazards, 2024 - Springer
Global climate change and land use alterations are contributing to more frequent and severe
extreme events globally. This trend is especially pronounced in the Loess Plateau's hilly …

De novo 2′‐fucosyllactose bioproduction through modular engineering of a novel Escherichia coli K12‐derived strain

C Ma, C Zhang, L Fan, C Deng, L Zhao - Food Bioengineering, 2023 - Wiley Online Library
The most abundant human milk oligosaccharide 2′‐fucosyllactose (2′‐FL) is a valuable
component that has gained significant attention from the food industry. To biosynthesize 2 …

Functional characterization of the Escherichia coli membrane transport protein MdtEF

C Tong, Y Liang, Q Liu, W Feng, H Yu, B Song - 2024 - researchsquare.com
The mdtE and mdtF genes encode membrane transporters, with their transport substrates
and mechanisms still not fully understood. Amino acid sequence analysis suggests that …