Leveraging the Power of Enzymes in Engineered Dead and Living Materials

MR Shannon, B Zhou… - Advanced Functional …, 2024 - Wiley Online Library
Significant advances are being made in the incorporation of enzymes into functional
materials, which leverage their inherent substrate specificity, efficient catalytic activity, and …

Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli

A Eskandari, NG Nezhad, TC Leow… - Archives of …, 2024 - Springer
Producing recombinant proteins is a major accomplishment of biotechnology in the past
century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of …

Chitinase and Deacetylase-Based Chitin-Degrading Bacteria: One-Pot Cascade Bioconversion of Chitin to Chitooligosaccharides

L Qing, J Gao, L Du, Y Liu, N Guo, J Sun… - Journal of Agricultural …, 2024 - ACS Publications
Cascade conversion of chitin into soluble and functional chitooligosaccharides has gained
great attention. However, the biotransformation route is still limited to the low catalytic …

Extracellular vesicles of the probiotic bacteria E. coli O83 activate innate immunity and prevent allergy in mice

AM Schmid, A Razim, M Wysmołek, D Kerekes… - Cell Communication and …, 2023 - Springer
Abstract Background E. coli O83 (Colinfant Newborn) is a Gram-negative (G-) probiotic
bacterium used in the clinic. When administered orally, it reduces allergic sensitisation but …

Boosting extracellular FastPETase production in E. coli: A combined approach of cognate chaperones co-expression and vesicle nucleating peptide tag fusion

T Wu, H Sun, W Wang, B Xie, Z Wang, J Lu, A Xu… - International Journal of …, 2024 - Elsevier
Enzymatic PET recycling has emerged as a promising green solution in addition to
mechanical recycling, but low soluble expression levels of the inherently hydrophobic PET …

Continuous-Flow microreactor enhanced biotransformation of linoleic acid using Lipoxygenase of catalytically-active inclusion body

CA Liu, R Gao, J Xiong, X Yuan, Y Cao… - Chemical Engineering …, 2025 - Elsevier
Enzymatic hydroxylation of unsaturated fatty acid using lipoxygenase (LOX) is an efficient
and sustainable pathway for the production of high value-added compounds. Difficulty in …

Membrane vesicle engineering with “à la carte” bacterial‐immunogenic molecules for organism‐free plant vaccination

I Jiménez‐Guerrero, FJ López‐Baena… - Microbial …, 2023 - Wiley Online Library
Abstract The United Nations heralds a world population exponential increase exceeding 9.7
billion by 2050. This poses the challenge of covering the nutritional needs of an …

Optimised production and analysis of recombinant protein-filled vesicles from E. coli.

B Streather, K Baker, T Eastwood… - Journal of Visualized …, 2023 - kar.kent.ac.uk
This protocol describes a detailed method for the bacterial production of recombinant
proteins, including typically insoluble or disulphide-bond containing proteins, packaged …

A Method for assessing the quality of recombinant human milk peptide analogues

AT Mekhtieva, AS Martynyuk… - …, 2024 - pharmacophorejournal.com
Recently researchers isolated and characterized proteolytic fragments of human kappa
casein with a molecular weight of~ 8.6 kDa from human milk [1]. Its genetically engineered …

The Production of Recombinant Proteins (Video)| JoVE

BR Streather, K Baker, TA Eastwood… - JoVE (Journal of …, 2023 - jove.com
This innovative system, using a short peptide tag, that exports multiple recombinant proteins
in membrane bound vesicles from E. coli, provides an effective solution to a range of …