Strategies for volumetric imaging with a fluorescence microscope

J Mertz - Optica, 2019 - opg.optica.org
Three-dimensional fluorescence imaging has been a longstanding goal for microscopists,
made all the more challenging when aiming for a trifecta of resolution, speed, and field of …

[HTML][HTML] Three-photon excited fluorescence imaging in neuroscience: From principles to applications

Y Xiao, P Deng, Y Zhao, S Yang, B Li - Frontiers in Neuroscience, 2023 - frontiersin.org
The development of three-photon microscopy (3PM) has greatly expanded the capability of
imaging deep within biological tissues, enabling neuroscientists to visualize the structure …

[HTML][HTML] High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

JL Fan, JA Rivera, W Sun, J Peterson… - Nature …, 2020 - nature.com
Understanding the structure and function of vasculature in the brain requires us to monitor
distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) …

Simultaneous multiplane imaging with reverberation two-photon microscopy

DR Beaulieu, IG Davison, K Kılıç, TG Bifano, J Mertz - Nature methods, 2020 - nature.com
Multiphoton microscopy has gained enormous popularity because of its unique capacity to
provide high-resolution images from deep within scattering tissue. Here, we demonstrate …

Two-photon Bessel beam tomography for fast volume imaging

AF Valle, JD Seelig - Optics express, 2019 - opg.optica.org
Light microscopy on dynamic samples, for example neural activity in the brain, often requires
imaging volumes that extend over several 100 µm in axial direction at a rate of at least …

Direct observation and characterization of optical guiding of microparticles by tightly focused non-diffracting beams

Y Liang, S Yan, B Yao, M Lei - Optics Express, 2019 - opg.optica.org
Due to the propagation-invariant and self-healing properties, nondiffracting beams are
highly attractive in optical trapping. However, little attention has been paid to investigating …

Shaping the on-axis intensity profile of generalized Bessel beams by iterative optimization methods

R Li, X Yu, T Peng, Y Yang, B Yao, C Zhang… - Journal of …, 2018 - iopscience.iop.org
The Bessel beam belongs to a typical class of non-diffractive optical fields that are
characterized by their invariant transverse profiles with the beam propagation. The extended …

High-efficiency generation of long-distance, tunable, high-order nondiffracting beams

X Yun, Y Liang, M He, L Guo, X Zhang, S Wang… - Photonics …, 2024 - opg.optica.org
Nondiffracting beams (NDBs) have presented significant utility across various fields for their
unique properties of self-healing, anti-diffraction, and high-localized intensity distribution …

Tunable depth of focus with modified complex amplitude modulation of an optical field

W Yin, Y Yang, R Yang, B Yao - Applied Optics, 2022 - opg.optica.org
Bessel beams have nondiffraction and self-healing properties in the propagation direction
and are widely used in particle optical manipulation and optical microscopy. Bessel beams …

A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscope

T Mo, Y Liu, F Bie, Z Dai, J Chang, H Gong… - Journal of Innovative …, 2023 - World Scientific
The volumetric imaging of two-photon microscopy expands the focal depth and improves the
throughput, which has unparalleled superiority for three-dimension samples, especially in …