Integration, excision, and inversion of defined DNA segments commonly occur through site-
specific recombination, a process of DNA breakage and reunion that requires no DNA …

Tyrosine-type site-specific recombinases (T-SSRs) have opened new avenues for the
predictable modification of genomes as they enable precise genome editing in heterologous …

The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert
DNA cassettes into eukaryotic chromosomes is widespread (1–9). Indeed, a PubMed search …

Conspectus Direct imaging of molecular motions is one of the most fundamental issues for
elucidating the physical properties of individual molecules and their reaction mechanisms …

Cre recombinase is a phage-derived enzyme that has found utility for precise manipulation
of DNA sequences. Cre recognizes and recombines pairs of loxP sequences characterized …

Site-specific recombinases such as the Cre-LoxP system are routinely used for genome
engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the …

Members of the tyrosine recombinase (YR) family of site-specific recombinases catalyze
DNA rearrangements using phosphoryl transfer chemistry that is identical to that used by the …

SUMMARY A classical feature of the tyrosine recombinase family of proteins catalyzing site-
specific recombination, as exemplified by the phage lambda integrase and the Cre and Flp …

The single-molecule fluorescence technique is becoming a general and mature tool to
probe interactions and dynamics of biomolecules with ultra high precision and accuracy …

Site-specific recombination involves reciprocal exchange between defined DNA sites. The
reaction initiates from the formation of a recombinase–DNA synaptic complex, in which two …