When phased without water: biophysics of cellular desiccation, from biomolecules to condensates

PS Romero-Perez, Y Dorone, E Flores… - Chemical …, 2023 - ACS Publications
The molecular machinery that enables life has evolved in water, yet many of the organisms
around us are able to survive even extreme desiccation. Especially remarkable are single …

Active glasses

LMC Janssen - Journal of Physics: Condensed Matter, 2019 - iopscience.iop.org
Active glassy matter has recently emerged as a novel class of non-equilibrium soft matter,
combining energy-driven, active particle movement with dense and disordered glass-like …

Protein diffusion in Escherichia coli cytoplasm scales with the mass of the complexes and is location dependent

WM Śmigiel, L Mantovanelli, DS Linnik, M Punter… - Science …, 2022 - science.org
We analyze the structure of the cytoplasm by performing single-molecule displacement
mapping on a diverse set of native cytoplasmic proteins in exponentially growing …

Sustained enzymatic activity and flow in crowded protein droplets

A Testa, M Dindo, AA Rebane, B Nasouri… - Nature …, 2021 - nature.com
Living cells harvest energy from their environments to drive the chemical processes that
enable life. We introduce a minimal system that operates at similar protein concentrations …

Cytoplasmic organization promotes protein diffusion in Xenopus extracts

WYC Huang, X Cheng, JE Ferrell Jr - Nature Communications, 2022 - nature.com
The cytoplasm is highly organized. However, the extent to which this organization influences
the dynamics of cytoplasmic proteins is not well understood. Here, we use Xenopus laevis …

Potential application of dynamic contrast enhanced ultrasound in predicting microvascular invasion of hepatocellular carcinoma

Y Dong, Y Qiu, D Yang, L Yu, D Zuo… - Clinical …, 2021 - content.iospress.com
OBJECTIVE: To investigate the clinical value of dynamic contrast enhanced ultrasound (D-
CEUS) in predicting the microvascular invasion (MVI) of hepatocellular carcinoma (HCC) …

Transcription and translation in cytomimetic protocells perform most efficiently at distinct macromolecular crowding conditions

MA Vibhute, MH Schaap, RJM Maas… - ACS Synthetic …, 2020 - ACS Publications
The formation of cytomimetic protocells that capture the physicochemical aspects of living
cells is an important goal in bottom-up synthetic biology. Here, we recreated the crowded …

Unjamming and emergent nonreciprocity in active ploughing through a compressible viscoelastic fluid

JP Banerjee, R Mandal, DS Banerjee… - Nature …, 2022 - nature.com
A dilute suspension of active Brownian particles in a dense compressible viscoelastic fluid,
forms a natural setting to study the emergence of nonreciprocity during a dynamical phase …

Macromolecular crowding: Sensing without a sensor

LJ Holt, M Delarue - Current Opinion in Cell Biology, 2023 - Elsevier
All living cells are crowded with macromolecules. Crowding can directly modulate
biochemical reactions to various degrees depending on the sizes, shapes, and binding …

Perspective: a stirring role for metabolism in cells

J Losa, S Leupold, D Alonso‐Martinez… - Molecular Systems …, 2022 - embopress.org
Based on recent findings indicating that metabolism might be governed by a limit on the rate
at which cells can dissipate Gibbs energy, in this Perspective, we propose a new …