Transcriptional profiling of somatostatin interneurons in the spinal dorsal horn

A Chamessian, M Young, Y Qadri, T Berta, RR Ji… - Scientific reports, 2018 - nature.com
The spinal dorsal horn (SDH) is comprised of distinct neuronal populations that process
different somatosensory modalities. Somatostatin (SST)-expressing interneurons in the SDH …

Isolated C. elegans germ nuclei exhibit distinct genomic profiles of histone modification and gene expression

M Han, G Wei, CE McManus, LDW Hillier, V Reinke - BMC genomics, 2019 - Springer
Background The wide variety of specialized permissive and repressive mechanisms by
which germ cells regulate developmental gene expression are not well understood genome …

Adipocyte nuclei captured from VAT and SAT

S Ambati, P Yu, EC McKinney, MK Kandasamy… - BMC obesity, 2016 - Springer
Background Obesity-related comorbidities are thought to result from the reprogramming of
the epigenome in numerous tissues and cell types, and in particular, mature adipocytes …

Genome-wide discovery of active regulatory elements and transcription factor footprints in Caenorhabditis elegans using DNase-seq

MCW Ho, P Quintero-Cadena… - Genome Research, 2017 - genome.cshlp.org
Deep sequencing of size-selected DNase I–treated chromatin (DNase-seq) allows high-
resolution measurement of chromatin accessibility to DNase I cleavage, permitting …

Laser microdissection for species-agnostic single-tissue applications

A Woronik, K Kiontke, RS Jallad… - Journal of …, 2022 - pmc.ncbi.nlm.nih.gov
Single-cell methodologies have revolutionized the analysis of the transcriptomes of specific
cell types. However, they often require species-specific genetic “toolkits,” such as promoters …

Expanding the Caenorhabditis elegans auxin-inducible degron system toolkit with internal expression and degradation controls and improved modular constructs for …

G Ashley, T Duong, MT Levenson, MAQ Martinez… - BioRxiv, 2020 - biorxiv.org
The auxin-inducible degron (AID) system has emerged as a powerful tool to conditionally
deplete proteins in a range of organisms and cell-types. Here, we describe a toolkit to …

Deciphering Precise Gene Transcriptional Expression Using gwINTACT in Tomato

Y Chu, J Gong, P Wu, Y Liu, Y Du, L Ma, D Fu… - Frontiers in Plant …, 2022 - frontiersin.org
Functional gene transcription mainly occurs in the nucleus and has a significant role in plant
physiology. The i solation of n uclei ta gged in specific c ell t ype (INTACT) technique …

Cell type‐specific epigenome profiling using affinity‐purified nuclei

Z Chitikova, FA Steiner - genesis, 2016 - Wiley Online Library
The development of a multicellular organism from a single zygote depends on the
differentiation of progenitor cells to specialized cell types. The differentiation of these cell …

A simple and effective method to isolate germ nuclei from C. elegans for genomic assays

M Han, G Wei, CE McManus, V Reinke - bioRxiv, 2018 - biorxiv.org
The wide variety of specialized permissive and repressive mechanisms by which germ cells
regulate developmental gene expression are not well understood genome-wide. Isolation of …

Netzwerk-und epigenetische Analyse eines Ultralangzeitgedächtnisses bei Drosophila melanogaster

L Spindler - 2019 - openscience.ub.uni-mainz.de
A fly, memorizing its body reach, adjusts its behavior at a gap according to the own body
size. Therefore, this fly will not try surmounting a gap that exceeds its reach by far. Formation …