Many biological processes are executed and regulated through the molecular interactions of
proteins and nucleic acids. Proximity labeling (PL) is a technology for tagging the …

There are inherent limitations with traditional methods to study protein behavior or to
determine the constituency of proteins in discrete subcellular compartments. In response to …

Trypanosoma brucei is a model trypanosomatid, an important group of human, animal and
plant unicellular parasites. Understanding their complex cell architecture and life cycle is …

The BioID method uses a promiscuous biotin ligase to detect protein–protein associations
as well as proximate proteins in living cells. Here we report improvements to the BioID …

Characterizing the proteome composition of organelles and subcellular regions of living
cells can facilitate the understanding of cellular organization as well as protein interactome …

Proximity‐dependent biotin identification (BioID) is a recently developed method that allows
the identification of proteins in the close vicinity of a protein of interest in living cells. BioID …

African trypanosomes are dixenous eukaryotic parasites that impose a significant human
and veterinary disease burden on sub-Saharan Africa. Diversity between species and life …

Background Photosynthetic euglenids are major contributors to fresh water ecosystems.
Euglena gracilis in particular has noted metabolic flexibility, reflected by an ability to thrive in …

A defining feature of Trypanosoma brucei cell shape is the lateral attachment of the
flagellum to the cell body, mediated by the flagellum attachment zone (FAZ). The FAZ is a …

The kinetoplastids Trypanosoma brucei and Leishmania mexicana are eukaryotes with a
highly structured cellular organisation that is reproduced with great fidelity in each …