MultiPrime: A reliable and efficient tool for targeted next‐generation sequencing

H Xia, Z Zhang, C Luo, K Wei, X Li, X Mu, M Duan… - iMeta, 2023 - Wiley Online Library
We present multiPrime, a novel tool that automatically designs minimal primer sets for
targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime …

Streamlined whole-genome genotyping through NGS-enhanced thermal asymmetric interlaced (TAIL)-PCR

S Zhao, Y Wang, Z Zhu, P Chen, W Liu, C Wang… - Plant …, 2024 - cell.com
Whole-genome genotyping (WGG) stands as a pivotal element in genomic-assisted plant
breeding. Nevertheless, sequencing-based approaches for WGG continue to be costly …

Genetic Diversity and Population Structure Analysis of Hollyhock (Alcea rosea Cavan) Using High-Throughput Sequencing

Y Wang, S Zhao, P Chen, Y Liu, Z Ma, WA Malik, Z Zhu… - Horticulturae, 2023 - mdpi.com
Hollyhock (Alcea rosea (Linn). Cavan) is an herbaceous flowering plant with significant
applications in urban greening, soil remediation, and traditional medicine. However, its …

Optimized semi-specific PCR amplification using arbitrarily degenerate primer for genome-wide genotyping and its application in peanut genetic diversity study

S Zhao, Y Wang, X Zhang, S Xie, H Chen, Y Yan, J Gu… - bioRxiv, 2024 - biorxiv.org
Cultivated peanut (Arachis hypogaea L.) is an important legume crop with a large genome
size but a limited genetic diversity. Evaluating the genetic variation among diverse peanut …