Opportunities and challenges of the tag-assisted protein purification techniques: Applications in the pharmaceutical industry

MM Gomari, N Saraygord-Afshari, M Farsimadan… - Biotechnology …, 2020 - Elsevier
Tag-assisted protein purification is a method of choice for both academic researches and
large-scale industrial demands. Application of the purification tags in the protein production …

Engineered living materials based on adhesin-mediated trapping of programmable cells

S Guo, E Dubuc, Y Rave, M Verhagen… - ACS synthetic …, 2020 - ACS Publications
Engineered living materials have the potential for wide-ranging applications such as
biosensing and treatment of diseases. Programmable cells provide the functional basis for …

Carbohydrate Research Mini-review Relationships between bacteria and the mucus layer

I Brockhausen, D Falconer, S Sara - Carbohydrate Research, 2024 - Elsevier
The mucus layer on epithelial cells is an essential barrier, as well as a nutrient-rich niche for
bacteria, forming a dynamic, functional and symbiotic ecosystem and first line of defense …

Sugar-binding and split domain combinations in repeats-in-toxin adhesins from Vibrio cholerae and Aeromonas veronii mediate cell-surface recognition and …

M Sherik, R Eves, S Guo, CJ Lloyd, KE Klose… - Mbio, 2024 - Am Soc Microbiol
Many pathogenic Gram-negative bacteria use repeats-in-toxin adhesins for colonization and
biofilm formation. In the cholera agent Vibrio cholerae, flagellar-regulated hemagglutinin A …

Structural basis of ligand selectivity by a bacterial Adhesin Lectin involved in multispecies biofilm formation

S Guo, TDR Vance, H Zahiri, R Eves, C Stevens… - MBio, 2021 - Am Soc Microbiol
Carbohydrate recognition by lectins governs critical host-microbe interactions. Mp PA14
(Marinomonas primoryensis PA14 domain) lectin is a domain of a 1.5-MDa adhesin …

[HTML][HTML] Essential role of calcium in extending RTX adhesins to their target

TDR Vance, Q Ye, B Conroy, PL Davies - Journal of Structural Biology: X, 2020 - Elsevier
RTX adhesins are long, multi-domain proteins present on the outer membrane of many
Gram-negative bacteria. From this vantage point, adhesins use their distal ligand-binding …

Adhesin domains responsible for binding bacteria to surfaces they colonize project outwards from companion split domains

LA Graham, T Hansen, Y Yang, M Sherik… - Proteins: Structure …, 2024 - Wiley Online Library
Bacterial adhesins attach their hosts to surfaces that the bacteria will colonize. This surface
adhesion occurs through specific ligand‐binding domains located towards the distal end of …

[HTML][HTML] Molecular basis for inhibition of adhesin-mediated bacterial-host interactions through a peptide-binding domain

S Guo, H Zahiri, C Stevens, DC Spaanderman… - Cell Reports, 2021 - cell.com
Infections typically begin with pathogens adhering to host cells. For bacteria, this adhesion
can occur through specific ligand-binding domains. We identify a 20-kDa peptide-binding …

Efficacy of EDTA-NS irrigation in eradicating Staphylococcus aureus biofilm-associated infection: an in vitro and in vivo study

J Lin, J Suo, B Bao, H Wei, T Gao, H Zhu… - Bone & Joint …, 2024 - boneandjoint.org.uk
Aims To investigate the efficacy of ethylenediaminetetraacetic acid-normal saline (EDTA-
NS) in dispersing biofilms and reducing bacterial infections. Methods EDTA-NS solutions …

Chengkuizengella axinellae sp. nov., a symbiotic bacterium isolated from a marine sponge of the genus Axinella

YL Moon, KH Kim, JS Park - Antonie van Leeuwenhoek, 2024 - Springer
A Gram-stain-positive, strictly aerobic, creamy-white colored, endospore-forming and non-
motile rods strain, designated as strain 2205SS18-9T, was isolated from a marine sponge …