Role of distal sites in enzyme engineering

J Gu, Y Xu, Y Nie - Biotechnology Advances, 2023 - Elsevier
The limitations associated with natural enzyme catalysis have triggered the rise of the field of
protein engineering. Traditional rational design was based on the analysis of protein …

Evaluating Multienzyme Cascade Routes for Pharmaceutically Relevant Molecules

K Naik, S Dheeraj, K Jeevani… - European Journal of …, 2024 - Wiley Online Library
The constant demand for sustainable approaches to pharmaceutical synthesis has thrived
the enzyme catalysis (biocatalysis) field. The most sought‐after features like high specificity …

Superior Proton‐Transfer Catalytic Promiscuity of Cytochrome c in Self‐Organized Media

S Rani, B Dasgupta, GK Bhati, K Tomar… - …, 2021 - Wiley Online Library
Evolutionarily elderly proteins commonly feature greater catalytic promiscuity. Cytochrome c
is among the first set of proteins in evolution to have known prospects in electron transport …

Semi‐rational engineering of an amino acid racemase that is stabilized in aqueous/organic solvent mixtures

C Femmer, M Bechtold, S Panke - Biotechnology and …, 2020 - Wiley Online Library
Enzymes are industrially applied under increasingly diverse environmental conditions that
are dictated by the efforts to optimize overall process efficiency. Engineering the operational …

Using mutability landscapes to guide enzyme thermostabilization

C Guo, Y Ni, L Biewenga, T Pijning… - …, 2021 - Wiley Online Library
Thermostabilizing enzymes while retaining their activity and enantioselectivity for applied
biocatalysis is an important topic in protein engineering. Rational and computational design …

Enantiocomplementary Michael Additions of Acetaldehyde to Aliphatic Nitroalkenes Catalyzed by Proline‐Based Carboligases

A Kunzendorf, M Saifuddin, GJ Poelarends - ChemBioChem, 2022 - Wiley Online Library
The blockbuster drug Pregabalin is widely prescribed for the treatment of painful diabetic
neuropathy. Given the continuous epidemic growth of diabetes, the development of …

Exploring Oxidoreductases from Extremophiles for Biosynthesis in a Non-Aqueous System

S Wang, H Lei, Z Ji - International Journal of Molecular Sciences, 2023 - mdpi.com
Organic solvent tolerant oxidoreductases are significant for both scientific research and
biomanufacturing. However, it is really challenging to obtain oxidoreductases due to the …