A double-mutant cycle involves wild-type protein, two single mutants and the corresponding
double mutant protein. If the change in free energy associated with a structural or functional …

Enzymes continue to be the subject of intensive research efforts because of their ability to
accelerate chemical reactions by factors as large as 1020 with extraordinary selectivity. 1 …

Polyubiquitin chains linked through Lys48 are the principal signal for targeting substrates to
the 26S proteasome. Through studies of structurally defined, polyubiquitylated model …

A network of coupled promoting motions in the enzyme dihydrofolate reductase is identified
and characterized. The present identification is based on genomic analysis for sequence …

Herpes simplex virus type 1 (HSV-1) thymidine kinase is currently used as a suicide agent in
the gene therapy of cancer. This therapy is based on the preferential phosphorylation of …

Nudix hydrolases catalyze the hydrolysis of nucleoside diphosphates linked to other
moieties, X, and contain the sequence motif or Nudix box, GX5EX7REUXEEXGU. The …

▪ Abstract Since the discovery of enzymes as biological catalysts, study of their enormous
catalytic power and exquisite specificity has been central to biochemistry. Nevertheless …

Additive or non‐additive, that is the question: The increasing awareness of non‐additive
cooperative mutational effects in protein engineering is currently providing new theoretical …

Most enzymes involved in cell signaling, such as protein kinases, protein phosphatases,
GTPases, and nucleotide cyclases catalyze nucleophilic substitutions at phosphorus. When …

Recently, the participation in catalysis of residues spatially removed from the enzyme's
active site has received considerable attention. The influence of the distal Gly-121 residue …