Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …

The central dogma of modern biology underscores the pivotal roles proteins play in diverse
biological processes, the study of which necessitates advanced methods to produce …

Chemoenzymatic protein and peptide modification is a powerful means of generating
defined, homogeneous conjugates for a range of applications. However, the use of …

Enzymatic peptide ligation holds great promise in the study of protein functions and
development of protein therapeutics. Owing to their high catalytic efficiency and a minimal …

The engineering of potent prophylactic and therapeutic complexes has always required
careful protein modification techniques with seamless capabilities. In this light, methods that …

Transpeptidase‐catalyzed protein and peptide modifications have been widely utilized for
generating conjugates of interest for biological investigation or therapeutic applications …

Transpeptidases are powerful tools for protein engineering but are largely restricted to
acting at protein backbone termini. Alternative enzymatic approaches for internal protein …

Peptide ligases are versatile enzymes that can be utilized for precise protein conjugation for
bioengineering applications. Hyperactive peptide asparaginyl ligases (PALs), such as …

Enzymes are widely used for protein ligation because of their efficient and site-specific
connections under mild conditions. However, many enzymatic ligations are restricted to …

Asparaginyl endopeptidases (AEPs) are cysteinyl enzymes naturally catalyzing the
hydrolysis and transpeptidation reactions at Asx–Xaa bonds. These reactions go by a …