The maturation of high-throughput short-read sequencing technology over the past two
decades has shaped the way genomes are studied. Recently, single-molecule, long-read …

The advent of single-molecule, long-read sequencing (LRS) technologies by Oxford
Nanopore Technologies and Pacific Biosciences has revolutionized genomics …

The short read-length of next-generation sequencing makes it challenging to characterize
highly repetitive regions (HRRs) such as centromeres, telomeres and ribosomal DNAs …

Over half of human genomic DNA is composed of repetitive sequences generated
throughout evolution by prolific mobile genetic parasites called transposable elements …

Epigenetic regulations, including chromatin accessibility, nucleosome positioning and DNA
methylation intricately shape genome function. However, current chromatin profiling …

Analysis of transcription factors and chromatin modifications at the genome-wide level
provides insights into gene regulatory processes, such as transcription, cell differentiation …

Nanopore sequencing maps biochemical processes on DNA by detecting negative peaks in
the sequence alignment profile. Protein-bound DNA and single-strand broken DNA cannot …

DNA-protein interactions are essential for genome functioning, with billions of years of
evolution shaping specific patterns of protein-DNA interactions to regulate gene networks in …

Genomic approaches have provided detailed insight into chromosome architecture.
However, commonly deployed techniques do not preserve connectivity-based information …

We recently developed directed methylation with long-read sequencing (DiMeLo-seq) to
map protein–DNA interactions genome wide. DiMeLo-seq is capable of mapping multiple …