Formation of mRNA 3′ ends in eukaryotes requires the interaction of transacting factors
with cis-acting signal elements on the RNA precursor by two distinct mechanisms, one for …

Abstract The RNA polymerase II CTD is essential for 3′ end cleavage of metazoan pre-
mRNAs and binds 3′ end processing factors in vitro. We show genetic and biochemical …

In yeast, four factors (CF I, CF II, PF I, and PAP) are required for accurate pre-mRNA
cleavage and polyadenylation in vitro. CF I can be separated further into CF IA and CF IB …

In Saccharomyces cerevisiae, the single poly (A) binding protein, Pab1, is the major
ribonucleoprotein associated with the poly (A) tails of mRNAs in both the nucleus and the …

CF II, a factor required for cleavage of the 3′ ends of mRNA precursor in Saccharomyces
cerevisiae, has been shown to contain four polypeptides. The three largest subunits …

In Saccharomyces cerevisiae, in vitro mRNA cleavage and polyadenylation require the poly
(A) binding protein, Pab1p, and two multiprotein complexes: CFI (cleavage factor I) and CPF …

Fip1 is an essential component of the Saccharomyces cerevisiae polyadenylation
machinery and the only protein known to interact directly with poly (A) polymerase (Pap1). Its …

The cleavage/polyadenylation factor I (CF I) is one of four factors required for mRNA 3′ end
formation in the yeast Saccharomyces cerevisiae. Here we describe the purification of CF I …

Background The identification of epitopes in proteins recognized by medically relevant
antibodies is useful for the development of peptide-based diagnostics and vaccines. In this …

Cleavage of pre-mRNA during 3′-end formation in yeast requires two protein factors,
cleavage factor I (CF I) and cleavage factor (CF II). A 5300-fold purification of CF II indicates …