Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow …

FA Ahmed, A Larrea-Sarmiento, AM Alvarez, M Arif - Scientific reports, 2018 - nature.com
Pectobacterium species cause serious bacterial soft rot diseases worldwide on
economically important fruit and vegetable crops including tomato and potato. Accurate and …

Multiplex recombinase polymerase amplification assay developed using unique genomic regions for rapid on-site detection of genus Clavibacter and C. nebraskensis

A Larrea-Sarmiento, JP Stack, AM Alvarez, M Arif - Scientific reports, 2021 - nature.com
Clavibacter is an agriculturally important bacterial genus comprising nine host-specific
species/subspecies including C. nebraskensis (Cn), which causes Goss's wilt and blight of …

A Novel LAMP Assay for the Detection of Phytophthora cinnamomi Utilizing a New Target Gene Identified From Genome Sequences

T Dai, X Yang, T Hu, Z Li, Y Xu, C Lu - Plant Disease, 2019 - Am Phytopath Society
Phytophthora cinnamomi is an ecologically and agriculturally significant plant pathogen.
Early and accurate detection of P. cinnamomi is paramount to disease prevention and …

New Approaches to Plant Pathogen Detection and Disease Diagnosis

J Cubero, PJ Zarco-Tejada… - …, 2024 - Am Phytopath Society
Detecting plant pathogens and diagnosing diseases are critical components of successful
pest management. These key areas have undergone significant advancements driven by …

Comparative genomics reveals signature regions used to develop a robust and sensitive multiplex TaqMan real‐time qPCR assay to detect the genus Dickeya and …

S Dobhal, G Boluk, B Babler, MJ Stulberg… - Journal of Applied …, 2020 - academic.oup.com
Aims Dickeya species are high consequence plant pathogenic bacteria; associated with
potato disease outbreaks and subsequent economic losses worldwide. Early, accurate and …

Genome-Informed Recombinase Polymerase Amplification Assay Coupled with a Lateral Flow Device for In-Field Detection of Dickeya Species

G Boluk, S Dobhal, AB Crockford, M Melzer… - Plant …, 2020 - Am Phytopath Society
Dickeya spp. cause blackleg and soft rot diseases of potato and several other plant species
worldwide, resulting in high economic losses. Rapid detection and identification of the …

Development of a loop‐mediated isothermal amplification assay for specific detection of all known subspecies of Clavibacter michiganensis

S Dobhal, A Larrea‐Sarmiento… - Journal of Applied …, 2019 - academic.oup.com
Aims Clavibacter michiganensis is an important bacterial plant pathogen that causes vast
destruction to agriculturally important crops worldwide. Early detection is critical to evaluate …

Rapid Detection of Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli in Common Bean by Loop-Mediated Isothermal Amplification

BAR de Paiva, A Wendland, NC Teixeira… - Plant …, 2020 - Am Phytopath Society
A single loop-mediated isothermal amplification (LAMP) assay was developed for specific
detection of both pathogens that cause bacterial blight in common bean, Xanthomonas …

Rapid Detection of Viral, Bacterial, Fungal, and Oomycete Pathogens on Tomatoes with Microneedles, LAMP on a Microfluidic Chip, and Smartphone Device

T Shymanovich, AC Saville, R Paul, Q Wei… - …, 2024 - Am Phytopath Society
Rapid detection of plant diseases before they escalate can improve disease control. Our
team has developed rapid nucleic acid extraction methods with microneedles and combined …

Specific colorimetric LAMP assay for the detection of maize bushy stunt phytoplasma in corn through comparative genomics

ICS Egito, A Rodrigues Alves, ICB Carvalho… - Tropical Plant …, 2024 - Springer
Brazil stands out as the third largest corn producer in the world, showing self-sufficiency to
supply its entire national demand. However, maize red stunt, caused by maize bushy stunt …