DNA damage and repair in plants–from models to crops
V Manova, D Gruszka - Frontiers in plant science, 2015 - frontiersin.org
The genomic integrity of every organism is constantly challenged by endogenous and
exogenous DNA-damaging factors. Mutagenic agents cause reduced stability of plant …
exogenous DNA-damaging factors. Mutagenic agents cause reduced stability of plant …
Use of designer nucleases for targeted gene and genome editing in plants
The ability to efficiently inactivate or replace genes in model organisms allowed a rapid
expansion of our understanding of many of the genetic, biochemical, molecular and cellular …
expansion of our understanding of many of the genetic, biochemical, molecular and cellular …
Both CRISPR/Cas‐based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana
Engineered nucleases can be used to induce site‐specific double‐strand breaks (DSB s) in
plant genomes. Thus, homologous recombination (HR) can be enhanced and targeted …
plant genomes. Thus, homologous recombination (HR) can be enhanced and targeted …
Synthetic nucleases for genome engineering in plants: prospects for a bright future
By inducing double‐strand breaks (DSB), it is possible to initiate DNA recombination. For a
long time, it was not possible to use DSB induction for efficient genome engineering due to …
long time, it was not possible to use DSB induction for efficient genome engineering due to …
Efficient in planta gene targeting in Arabidopsis using egg cell‐specific expression of the Cas9 nuclease of Staphylococcus aureus
Gene targeting (GT), the programmed change of genomic sequences by homologous
recombination (HR), is still a major challenge in plants. We previously developed an in …
recombination (HR), is still a major challenge in plants. We previously developed an in …
Site-directed nucleases: a paradigm shift in predictable, knowledge-based plant breeding
Conventional plant breeding exploits existing genetic variability and introduces new
variability by mutagenesis. This has proven highly successful in securing food supplies for …
variability by mutagenesis. This has proven highly successful in securing food supplies for …
[PDF][PDF] Gene targeting in plants: 25 years later
Only five years after the initiation of transgenic research in plants, gene targeting (GT) was
achieved for the first time in tobacco. Unfortunately, the frequency of targeted integration via …
achieved for the first time in tobacco. Unfortunately, the frequency of targeted integration via …
Opportunities and challenges with CRISPR-Cas mediated homologous recombination based precise editing in plants and animals
Key message We summarise recent advancements to achieve higher homologous
recombination based gene targeting efficiency in different animals and plants. Abstract The …
recombination based gene targeting efficiency in different animals and plants. Abstract The …
Genome editing and beyond: what does it mean for the future of plant breeding?
Main conclusion Genome editing offers revolutionized solutions for plant breeding to sustain
food production to feed the world by 2050. Therefore, genome-edited products are …
food production to feed the world by 2050. Therefore, genome-edited products are …
Meiotic recombination in Arabidopsis is catalysed by DMC1, with RAD51 playing a supporting role
O Da Ines, F Degroote, C Goubely, S Amiard… - PLoS …, 2013 - journals.plos.org
Recombination establishes the chiasmata that physically link pairs of homologous
chromosomes in meiosis, ensuring their balanced segregation at the first meiotic division …
chromosomes in meiosis, ensuring their balanced segregation at the first meiotic division …