To fully understand biological processes from the metabolism of a bacterium to the operation
of a human brain, it is necessary to know the three-dimensional (3D) spatial arrangement …

Highlights•Cryo-ET and subtomogram averaging allow visualizing macromolecular
complexes in close to physiological conditions.•This offers a significant potential for in situ …

AreTomo, an abbreviation for Alignment and Reconstruction for Electron Tomography, is a
GPU accelerated software package that fully automates motion-corrected marker-free …

Automated tomographic reconstruction is now possible in the IMOD software package,
including the merging of tomograms taken around two orthogonal axes. Several …

Abstract RNA polymerase I (Pol I) is a highly processive enzyme that transcribes ribosomal
DNA (rDNA) and regulates growth of eukaryotic cells,,,. Crystal structures of free Pol I from …

During starvation in the yeast Saccharomyces cerevisiae vacuolar vesicles fuse and lipid
droplets (LDs) can become internalized into the vacuole in an autophagic process named …

As x-ray and electron tomography is pushed further into the nanoscale, the limitations of
rotation stages become more apparent, leading to challenges in the alignment of the …

Electron tomography (ET) has emerged as a powerful technique to address fundamental
questions in molecular and cellular biology. It makes possible visualization of the molecular …

Background Tilt series are commonly used in electron tomography as a means of collecting
three-dimensional information from two-dimensional projections. A common problem …

During the last six decades electron microscopy (EM) has been essential to ultra-structural
studies of the cell to understand the fundamentals of cellular morphology and processes …