Introduction of a recombineering oligonucleotide and a CRISPR–Cas9 gene-targeting plasmid into Staphylococcus aureus for generating a gene-deletion strain
A Gründling, SJ Salipante - Cold Spring Harbor Protocols, 2024 - cshprotocols.cshlp.org
Gene deletions can be generated in Staphylococcus aureus using recombineering in
combination with a CRISPR–Cas9 counterselection approach. The method involves first …
combination with a CRISPR–Cas9 counterselection approach. The method involves first …
Allelic-exchange procedure in Staphylococcus aureus
MS Zeden, CF Schuster… - Cold Spring Harbor …, 2023 - cshprotocols.cshlp.org
This protocol continues a series of methods for the construction of an in-frame gene deletion
in Staphylococcus aureus strain RN4220. To this end, we describe in this protocol an allelic …
in Staphylococcus aureus strain RN4220. To this end, we describe in this protocol an allelic …
Cold Spring Harbor Laboratory Courses in Phage and Bacterial Genetics: A Catalyst for Innovation in Molecular Biology, Genetics, and Microbiology
S Maloy - Cold Spring Harbor Protocols, 2024 - cshprotocols.cshlp.org
The ability to answer complex scientific questions depends on the experimental methods
available. New methods often allow scientists to answer questions that were previously …
available. New methods often allow scientists to answer questions that were previously …
Introduction of a CRISPR–nCas9 Gene-Targeting Plasmid into Staphylococcus aureus for Gene Disruption
A Gründling, Q Ji - Cold Spring Harbor Protocols, 2024 - cshprotocols.cshlp.org
Methods for gene disruption are essential for functional genomics, and there are multiple
approaches for altering gene function in bacteria. One of these methods involves introducing …
approaches for altering gene function in bacteria. One of these methods involves introducing …