Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer
BACKGROUND Biomolecular mechanisms are typically inferred from static structural
“snapshots” obtained by x-ray crystallography, nuclear magnetic resonance (NMR) …
“snapshots” obtained by x-ray crystallography, nuclear magnetic resonance (NMR) …
Interaction dynamics of intrinsically disordered proteins from single-molecule spectroscopy
A Chowdhury, D Nettels… - Annual review of …, 2023 - annualreviews.org
Many proteins contain large structurally disordered regions or are entirely disordered under
physiological conditions. The functions of these intrinsically disordered proteins (IDPs) often …
physiological conditions. The functions of these intrinsically disordered proteins (IDPs) often …
Live imaging of SARS-CoV-2 infection in mice reveals that neutralizing antibodies require Fc function for optimal efficacy
Neutralizing antibodies (NAbs) are effective in treating COVID-19, but the mechanism of
immune protection is not fully understood. Here, we applied live bioluminescence imaging …
immune protection is not fully understood. Here, we applied live bioluminescence imaging …
Single-molecule FRET imaging of GPCR dimers in living cells
WB Asher, P Geggier, MD Holsey, GT Gilmore… - Nature …, 2021 - nature.com
Class CG protein-coupled receptors (GPCRs) are known to form stable homodimers or
heterodimers critical for function, but the oligomeric status of class A and B receptors, which …
heterodimers critical for function, but the oligomeric status of class A and B receptors, which …
lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription
RNA polymerase I (Pol I) transcription takes place at the border of the fibrillar center (FC)
and the dense fibrillar component (DFC) in the nucleolus. Here, we report that individual …
and the dense fibrillar component (DFC) in the nucleolus. Here, we report that individual …
[HTML][HTML] Real-time conformational dynamics of SARS-CoV-2 spikes on virus particles
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) mediates
viral entry into cells and is critical for vaccine development against coronavirus disease …
viral entry into cells and is critical for vaccine development against coronavirus disease …
Structural basis and mode of action for two broadly neutralizing antibodies against SARS-CoV-2 emerging variants of concern
Emerging variants of concern for the severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) can transmit more efficiently and partially evade protective immune …
(SARS-CoV-2) can transmit more efficiently and partially evade protective immune …
[HTML][HTML] CFTR function, pathology and pharmacology at single-molecule resolution
The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel that
regulates salt and fluid homeostasis across epithelial membranes. Alterations in CFTR …
regulates salt and fluid homeostasis across epithelial membranes. Alterations in CFTR …
Single-molecule imaging reveals allosteric stimulation of SARS-CoV-2 spike receptor binding domain by host sialic acid
Conformational dynamics of the severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) spike glycoprotein (S) mediate exposure of the binding site for the cellular receptor …
CoV-2) spike glycoprotein (S) mediate exposure of the binding site for the cellular receptor …
Structural basis of early translocation events on the ribosome
Peptide-chain elongation during protein synthesis entails sequential aminoacyl-tRNA
selection and translocation reactions that proceed rapidly (2–20 per second) and with a low …
selection and translocation reactions that proceed rapidly (2–20 per second) and with a low …