The recent boom in microfluidics and combinatorial indexing strategies, combined with low
sequencing costs, has empowered single-cell sequencing technology. Thousands—or even …

Rapid progress in the development of next-generation sequencing (NGS) technologies in
recent years has provided many valuable insights into complex biological systems, ranging …

Across the animal kingdom, gastrulation represents a key developmental event during which
embryonic pluripotent cells diversify into lineage-specific precursors that will generate the …

We perform a thorough analysis of RNA velocity methods, with a view towards
understanding the suitability of the various assumptions underlying popular …

Trajectory inference approaches analyze genome-wide omics data from thousands of single
cells and computationally infer the order of these cells along developmental trajectories …

Stroma is a poorly defined non-parenchymal component of virtually every organ with key
roles in organ development, homeostasis, and repair. Studies of the bone marrow stroma …

RNA abundance is a powerful indicator of the state of individual cells. Single-cell RNA
sequencing can reveal RNA abundance with high quantitative accuracy, sensitivity and …

INTRODUCTION During tissue turnover, stem and progenitor cells differentiate to produce
mature cell types. To understand and ultimately control differentiation, it is important to …

INTRODUCTION Metazoan development represents a big jump in complexity compared
with unicellular life in two aspects: cell-type differentiation and cell spatial organization. In …

Precise temporal control of gene expression in neuronal progenitors is necessary for correct
regulation of neurogenesis and cell fate specification. However, the cellular heterogeneity of …