[HTML][HTML] High-content CRISPR screening
CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …
interrogation of gene function in a wide range of applications and species. In pooled …
CRISPR in cancer biology and therapy
Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …
transforming biomedical research and providing entirely new approaches for dissecting all …
Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
Manipulation of DNA by CRISPR-Cas enzymes requires the recognition of a protospacer-
adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove …
adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove …
TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion
Abstract Exhausted CD8+ T (Tex) cells in chronic infections and cancer have limited effector
function, high co-expression of inhibitory receptors and extensive transcriptional changes …
function, high co-expression of inhibitory receptors and extensive transcriptional changes …
Chronos: a cell population dynamics model of CRISPR experiments that improves inference of gene fitness effects
CRISPR loss of function screens are powerful tools to interrogate biology but exhibit a
number of biases and artifacts that can confound the results. Here, we introduce Chronos …
number of biases and artifacts that can confound the results. Here, we introduce Chronos …
[HTML][HTML] RNA N6-methyladenosine methyltransferase METTL3 facilitates colorectal cancer by activating the m6A-GLUT1-mTORC1 axis and is a therapeutic target
Background & Aims RNA N 6-methyladenosine (m 6 A) modification has recently emerged
as a new regulatory mechanism in cancer progression. We aimed to explore the role of the …
as a new regulatory mechanism in cancer progression. We aimed to explore the role of the …
Promoter-bound METTL3 maintains myeloid leukaemia by m6A-dependent translation control
Abstract N 6-methyladenosine (m6A) is an abundant internal RNA modification in both
coding and non-coding RNAs, that is catalysed by the METTL3–METTL14 methyltransferase …
coding and non-coding RNAs, that is catalysed by the METTL3–METTL14 methyltransferase …
The N6-methyladenosine (m6A)-forming enzyme METTL3 controls myeloid differentiation of normal hematopoietic and leukemia cells
Abstract N 6-methyladenosine (m6A) is an abundant nucleotide modification in mRNA that is
required for the differentiation of mouse embryonic stem cells. However, it remains unknown …
required for the differentiation of mouse embryonic stem cells. However, it remains unknown …
Development of targeted protein degradation therapeutics
PP Chamberlain, LG Hamann - Nature chemical biology, 2019 - nature.com
Targeted protein degradation as a therapeutic modality has seen dramatic progress and
massive investment in recent years because of the convergence of two key scientific …
massive investment in recent years because of the convergence of two key scientific …
Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer
Pancreatic stellate cells (PSCs) differentiate into cancer-associated fibroblasts (CAFs) that
produce desmoplastic stroma, thereby modulating disease progression and therapeutic …
produce desmoplastic stroma, thereby modulating disease progression and therapeutic …