Origin of iodine preferential attack at sulfur in phosphorothioate and subsequent PO or PS bond dissociation

Q Huang, GY Lee, J Li, C Wang… - Proceedings of the …, 2022 - National Acad Sciences
Iodine-induced cleavage at phosphorothioate DNA (PT-DNA) is characterized by extremely
high sensitivity (∼ 1 phosphorothioate link per 106 nucleotides), which has been used for …

Understanding base and backbone contributions of phosphorothioate DNA for molecular recognition with SBD proteins

J Li, S Luo, X Ouyang, G Wu, Z Deng, X He… - Physical Chemistry …, 2023 - pubs.rsc.org
Bacterial DNA phosphorothioate (PT) modification provides a specific anchoring site for
sulfur-binding proteins (SBDs). Besides, their recognition patterns include phosphate links …

Molecular recognition between bacterial phosphorothioate DNA and sulfur-binding domain (SBD): competition between the water cage and chalcogen-hydrophobic …

J Li, H Wan, H Zhang, XL Wang, G Liu, G Wu… - Physical Chemistry …, 2022 - pubs.rsc.org
Bacterial DNA phosphorothioation (PT) physiologically and stereo-specifically replaces a
non-bridging oxygen in a phosphate link with a sulfur atom, which can be recognized by a …

Experimental and theoretical investigation on interactions between xylose-containing hemicelluloses and procyanidins

X Liu, J Li, A Rolland-Sabaté, S Perez… - Carbohydrate …, 2022 - Elsevier
During processing of plant-based foods, cell wall polysaccharides and polyphenols, such as
procyanidins, interact extensively, thereby affecting their physicochemical properties along …

Phosphorothioate-DNA bacterial diet reduces the ROS levels in C. elegans while improving locomotion and longevity

Q Huang, R Li, T Yi, F Cong, D Wang, Z Deng… - Communications …, 2021 - nature.com
DNA phosphorothioation (PT) is widely distributed in the human gut microbiome. In this
work, PT-diet effect on nematodes was studied with PT-bioengineering bacteria. We found …

Visualization of DNA damage and protection by atomic force microscopy in liquid

T Dai, Y Wang, G Yang - International Journal of Molecular Sciences, 2022 - mdpi.com
DNA damage is closely related to cancer and many aging-related diseases. Peroxynitrite is
a strong oxidant, thus a typical DNA damage agent, and is a major mediator of the …

Temporal dynamics and metagenomics of phosphorothioate epigenomes in the human gut microbiome

SR Byrne, MS DeMott, Y Yuan… - …, 2024 - pmc.ncbi.nlm.nih.gov
Background. Epigenetic regulation of gene expression and host defense is well established
in microbial communities, with dozens of DNA modifications comprising the epigenomes of …

Low energy photoionization of Phosphorothioate DNA-oligomers and ensuing hole transfer

Z Hu, Q Zhou, Z Jiao, P Qin, F Wang, Y Xia… - The Journal of …, 2022 - ACS Publications
Phosphorothioate (PS) modified oligonucleotides (S-DNA) naturally exist in bacteria and
archaea genome and are widely used as an antisense strategy in gene therapy. However …

[HTML][HTML] Proof of ssDNA degraded from dsDNA for ET recombination

Y Zheng, Y Zhang, X Li, L Liu - Biochemistry and Biophysics Reports, 2024 - Elsevier
The widely used ET recombination requires an ssDNA product degraded by Rac phage
protein E588 from dsDNA for strand invasion. However, proof of the ssDNA product is still …

Screening for Bongkrekic Acid in Food Using a Monoclonal Antibody-Based Indirect Competitive Enzyme-Linked Immunosorbent Assay (icELISA)

HL Wu, J Chen, F Pan, QS Xia, SW Zhang… - Analytical …, 2024 - Taylor & Francis
Pseudomonas cocovenenans subsp. farinofermentans is a pathogenic bacterium that
causes the poisoning of fermented rice products. Its isomeric products, bongkrekic acid (BA) …