Recent advances in biocatalysis with chemical modification and expanded amino acid alphabet

AD Pagar, MD Patil, DT Flood, TH Yoo… - Chemical …, 2021 - ACS Publications
The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

Enzyme immobilization onto the nanomaterials: Application in enzyme stability and prodrug-activated cancer therapy

M Sharifi, MJ Sohrabi, SH Hosseinali, A Hasan… - International Journal of …, 2020 - Elsevier
Nanoparticles (NPs) have been widely used for immobilization of wide ranges of enzymes.
However, the stabilization of enzymes on NPs is a major challenge, crucial for regulating …

Novozym 435: the “perfect” lipase immobilized biocatalyst?

C Ortiz, ML Ferreira, O Barbosa… - Catalysis Science & …, 2019 - pubs.rsc.org
Novozym 435 (N435) is a commercially available immobilized lipase produced by
Novozymes. It is based on immobilization via interfacial activation of lipase B from Candida …

Introducing a covalent thiol-based protected immobilized acetylcholinesterase with enhanced enzymatic performances for biosynthesis of esters

SRH Jangi, M Akhond - Process Biochemistry, 2022 - Elsevier
Acetylcholinesterase covalent immobilization was performed on mercaptoacetic acid-
functionalized magnetic nanoparticles. Upon the thiol-based protection of the enzyme active …

Taguchi design-assisted co-immobilization of lipase A and B from Candida antarctica onto chitosan: Characterization, kinetic resolution application, and docking …

KS Moreira, ALB de Oliveira… - … Research and Design, 2022 - Elsevier
In the present communication, the simultaneous co-immobilization by covalent binding of
lipase A from Candida antarctica (CALA) and lipase B from Candida antarctica (CALB) in …

Efficient hydrolysis of starch by α-amylase immobilized on cloisite 30B and modified forms of cloisite 30B by adsorption and covalent methods

H Aghaei, Z Mohammadbagheri, A Hemasi… - Food Chemistry, 2022 - Elsevier
In this paper, α-amylase from Bacillus subtilis was successfully immobilized on three
supports. First, α-amylase was immobilized on cloisite 30B via the adsorption method. Then …

Make proper surfaces for immobilization of enzymes: immobilization of lipase and α-amylase on modified Na-sepiolite

S Mortazavi, H Aghaei - International Journal of Biological Macromolecules, 2020 - Elsevier
This paper has focused on making suitable carriers for the immobilization of α-amylase from
Bacillus subtilis and lipase from Candida rugosa via adsorption on modified Na-sepiolite …

[HTML][HTML] Design of immobilized enzyme biocatalysts: Drawbacks and opportunities

CLB Reis, EYA Sousa, JF Serpa, RC Oliveira… - Química …, 2019 - SciELO Brasil
The enzymatic processes are increasingly highlights, especially in the synthesis of chemical
products with high added value. The enzyme immobilization can improve industrial …

Designing of nanomaterials-based enzymatic biosensors: Synthesis, properties, and applications

FTT Cavalcante, IR de A. Falcão, JE da S. Souza… - Electrochem, 2021 - mdpi.com
Among the many biological entities employed in the development of biosensors, enzymes
have attracted the most attention. Nanotechnology has been fostering excellent prospects in …

Lipase From Rhizomucor miehei Immobilized on Magnetic Nanoparticles: Performance in Fatty Acid Ethyl Ester (FAEE) Optimized Production by the Taguchi Method

KS Moreira, ALB de Oliveira, LSM Júnior… - … in Bioengineering and …, 2020 - frontiersin.org
In this communication, it was evaluated the production of fatty acid ethyl ester (FAAE) from
the free fatty acids of babassu oil catalyzed by lipase from Rhizomucor miehei (RML) …