Discovery of digestive enzymes in carnivorous plants with focus on proteases

R Ravee, FIM Salleh, HH Goh - PeerJ, 2018 - peerj.com
Background Carnivorous plants have been fascinating researchers with their unique
characters and bioinspired applications. These include medicinal trait of some carnivorous …

Nepenthes: State of the art of an inspiring plant for biotechnologists

S Miguel, A Hehn, F Bourgaud - Journal of biotechnology, 2018 - Elsevier
Plant carnivory results from the adaptation of plants to their environment. The capture and
digestion of preys, followed by their assimilation by the plant is a source of additional …

Addressing proteolytic efficiency in enzymatic degradation therapy for celiac disease

M Rey, M Yang, L Lee, Y Zhang, JG Sheff… - Scientific reports, 2016 - nature.com
Celiac disease is triggered by partially digested gluten proteins. Enzyme therapies that
complete protein digestion in vivo could support a gluten-free diet, but the barrier to …

Carnivorous Nutrition in Pitcher Plants (Nepenthes spp.) via an Unusual Complement of Endogenous Enzymes

L Lee, Y Zhang, B Ozar, CW Sensen… - Journal of proteome …, 2016 - ACS Publications
Plants belonging to the genus Nepenthes are carnivorous, using specialized pitfall traps
called “pitchers” that attract, capture, and digest insects as a primary source of nutrients. We …

Aspartic protease nepenthesin-1 as a tool for digestion in hydrogen/deuterium exchange mass spectrometry

A Kadek, H Mrazek, P Halada, M Rey… - Analytical …, 2014 - ACS Publications
Hydrogen/deuterium exchange coupled to mass spectrometry (HXMS) utilizes enzymatic
digestion of proteins to localize the information about altered exchange patterns in protein …

Recombinant nepenthesin II for hydrogen/deuterium exchange mass spectrometry

M Yang, M Hoeppner, M Rey, A Kadek, P Man… - Analytical …, 2015 - ACS Publications
The pitcher secretions of the Nepenthes genus of carnivorous plants contain a proteolytic
activity that is very useful for hydrogen/deuterium exchange mass spectrometry (HX-MS) …

Proteome analysis of digestive fluids in Nepenthes pitchers

S Rottloff, S Miguel, F Biteau, E Nisse… - Annals of …, 2016 - academic.oup.com
Abstract Background and Aims Carnivorous plants have developed strategies to enable
growth in nutrient-poor soils. For the genus Nepenthes, this strategy represents producing …

Nepenthesin Protease Activity Indicates Digestive Fluid Dynamics in Carnivorous Nepenthes Plants

F Buch, WE Kaman, FJ Bikker, A Yilamujiang… - PLoS …, 2015 - journals.plos.org
Carnivorous plants use different morphological features to attract, trap and digest prey,
mainly insects. Plants from the genus Nepenthes possess specialized leaves called pitchers …

Atypical and nucellin-like aspartic proteases: emerging players in plant developmental processes and stress responses

A Soares, SM Ribeiro Carlton… - Journal of experimental …, 2019 - academic.oup.com
Members of the pepsin-like family (A1) of aspartic proteases (APs) are widely distributed in
plants. A large number of genes encoding putative A1 APs are found in different plant …

Thiol-ene monolithic pepsin microreactor with a 3D-printed interface for efficient UPLC-MS peptide mapping analyses

A Jönsson, RR Svejdal, N Bøgelund… - Analytical …, 2017 - ACS Publications
To improve the sample handling, and reduce cost and preparation time, of peptide mapping
LC-MS workflows in protein analytical research, we here investigate the possibility of …