The first wave of genetically targeted therapies for cancer focused on drugging gene
products that are recurrently mutated in specific cancer types. However, mutational analysis …

A synthetic lethal interaction occurs between two genes when the perturbation of either gene
alone is viable but the perturbation of both genes simultaneously results in the loss of …

Protein‐protein interaction plays key role in predicting the protein function of target protein
and drug ability of molecules. The majority of genes and proteins realize resulting …

INTRODUCTION Genetic interactions occur when mutations in different genes combine to
result in a phenotype that is different from expectation based on those of the individual …

Genetic interactions identify combinations of genetic variants that impinge on phenotype.
With whole-genome sequence information available for thousands of individuals within a …

In this essay, we revisit the status of yeast as a model system for biology. We first summarize
important contributions of yeast to eukaryotic biology that we anticipated in 1988 in our first …

The classical genetic approach for exploring biological pathways typically begins by
identifying mutations that cause a phenotype of interest. Overexpression or misexpression of …

We have constructed and tested a dominant resistance module, for selection of S. cerevisiae
transformants, which entirely consists of heterologous DNA. This kanMX module contains …

Two genes are synthetic lethal if mutation of either alone is compatible with viability but
mutation of both leads to death. So, targeting a gene that is synthetic lethal to a cancer …

The function and activity of a protein are often modulated by other proteins with which it
interacts. This review is intended as a practical guide to the analysis of such protein-protein …