A short-chain acyl-CoA synthetase that supports branched-chain fatty acid synthesis in Staphylococcus aureus

SG Whaley, MW Frank, CO Rock - Journal of Biological Chemistry, 2023 - ASBMB
Staphylococcus aureus controls its membrane biophysical properties using branched-chain
fatty acids (BCFAs). The branched-chain acyl-CoA precursors, utilized to initiate fatty acid …

A temperature-sensitive metabolic valve and a transcriptional feedback loop drive rapid homeoviscous adaptation in Escherichia coli

L Hoogerland, SPH van den Berg, Y Suo… - Nature …, 2024 - nature.com
All free-living microorganisms homeostatically maintain the fluidity of their membranes by
adapting lipid composition to environmental temperatures. Here, we quantify enzymes and …

Malonyl-acyl carrier protein decarboxylase activity promotes fatty acid and cell envelope biosynthesis in Proteobacteria

SG Whaley, CD Radka, C Subramanian… - Journal of Biological …, 2021 - ASBMB
Bacterial fatty acid synthesis in Escherichia coli is initiated by the condensation of an acetyl-
CoA with a malonyl-acyl carrier protein (ACP) by the β-ketoacyl-ACP synthase III enzyme …

Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation

JP Prince, JR Bolla, GLM Fisher, J Mäkelä… - Nature …, 2021 - nature.com
Abstract Structural Maintenance of Chromosomes (SMC) complexes act ubiquitously to
compact DNA linearly, thereby facilitating chromosome organization-segregation. SMC …

Posttranslational control of PlsB is sufficient to coordinate membrane synthesis with growth in Escherichia coli

MJ Noga, F Büke, NJF van den Broek, NCE Imholz… - Mbio, 2020 - Am Soc Microbiol
Every cell must produce enough membrane to contain itself. However, the mechanisms by
which the rate of membrane synthesis is coupled with the rate of cell growth remain …

PlsX and PlsY: Additional roles beyond glycerophospholipid synthesis in Gram-negative bacteria

AN Rex, BW Simpson, G Bokinsky, MS Trent - mBio, 2024 - journals.asm.org
The unique asymmetry of the Gram-negative outer membrane, with glycerophospholipids
(GPLs) in the inner leaflet and lipopolysaccharide (LPS) in the outer leaflet, works to resist …

Loss of YhcB results in overactive fatty acid biosynthesis

HM Stanley, MS Trent - Mbio, 2024 - Am Soc Microbiol
Loss of the Escherichia coli inner membrane protein YhcB results in pleomorphic cell
morphology and clear growth defects. Prior work suggested that YhcB was directly involved …

Comparative proteomic analysis of Escherichia coli O157: H7 following ohmic and water bath heating by capillary-HPLC-MS/MS

X Tian, Q Yu, W Wu, X Li, R Dai - International journal of food microbiology, 2018 - Elsevier
Escherichia coli O157: H7 is an important food-borne pathogenic microorganism that has
been used as a model organism for studying microbial inactivation effects and inactivation …

Evaluation of mechanical features and antibacterial potential of fluoroquinolone against β-ketoacyl-ACP synthases (FabB, FabF & FabH) via computational …

SA Ejaz, M Aziz, TA Wani, HM Al-Kahtani - Archives of Biochemistry and …, 2023 - Elsevier
The synthesis of fatty acids, which are essential for the growth and survival of bacterial cells,
is catalyzed by beta-keto acyl-ACP synthase I-III. Due to the significant differences between …

Exogenous fatty acids inhibit fatty acid synthesis through competition between endogenously-and exogenously-generated substrates for phospholipid synthesis in …

SPH van den Berg, A Zoumaro-Djayoon, F Yang… - bioRxiv, 2024 - biorxiv.org
Exogenous fatty acids are directly incorporated into bacterial membranes, heavily
influencing bacterial ecology and antibiotic susceptibility. We use liquid …