Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges
L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
Nuclear pores as versatile reference standards for quantitative superresolution microscopy
Quantitative fluorescence and superresolution microscopy are often limited by insufficient
data quality or artifacts. In this context, it is essential to have biologically relevant control …
data quality or artifacts. In this context, it is essential to have biologically relevant control …
Systematic nanoscale analysis of endocytosis links efficient vesicle formation to patterned actin nucleation
Clathrin-mediated endocytosis is an essential cellular function in all eukaryotes that is driven
by a self-assembled macromolecular machine of over 50 different proteins in tens to …
by a self-assembled macromolecular machine of over 50 different proteins in tens to …
Visualizing the functional architecture of the endocytic machinery
Clathrin-mediated endocytosis is an essential process that forms vesicles from the plasma
membrane. Although most of the protein components of the endocytic protein machinery …
membrane. Although most of the protein components of the endocytic protein machinery …
Maximum-likelihood model fitting for quantitative analysis of SMLM data
Quantitative data analysis is important for any single-molecule localization microscopy
(SMLM) workflow to extract biological insights from the coordinates of the single …
(SMLM) workflow to extract biological insights from the coordinates of the single …
Direct comparison of clathrin-mediated endocytosis in budding and fission yeast reveals conserved and evolvable features
Conserved proteins drive clathrin-mediated endocytosis (CME), which from yeast to humans
involves a burst of actin assembly. To gain mechanistic insights into this process, we …
involves a burst of actin assembly. To gain mechanistic insights into this process, we …
Type-I myosins promote actin polymerization to drive membrane bending in endocytosis
Clathrin-mediated endocytosis in budding yeast requires the formation of a dynamic actin
network that produces the force to invaginate the plasma membrane against the intracellular …
network that produces the force to invaginate the plasma membrane against the intracellular …
Self-organizing silver and ultrasmall iron oxide nanoparticles prepared with ginger rhizome extract: Characterization, biomedical potential and microstructure analysis …
O Ivashchenko, J Gapiński, B Peplińska, Ł Przysiecka… - Materials & Design, 2017 - Elsevier
Multimodal nanoparticles (NPs) that may be used for therapies and diagnostics is the most
promising trend for efficient therapy. We demonstrate that nanocomposite based on self …
promising trend for efficient therapy. We demonstrate that nanocomposite based on self …
High-throughput ultrastructure screening using electron microscopy and fluorescent barcoding
Genetic screens using high-throughput fluorescent microscopes have generated large
datasets, contributing many cell biological insights. Such approaches cannot tackle …
datasets, contributing many cell biological insights. Such approaches cannot tackle …
Optimized sample preparation for single-molecule localization-based superresolution microscopy in yeast
C Kaplan, H Ewers - Nature protocols, 2015 - nature.com
Single-molecule localization-based superresolution microscopy methods allow the
resolution of cellular structures in the range of tens of nanometers. However, these …
resolution of cellular structures in the range of tens of nanometers. However, these …