With the advent of directed evolution techniques, protein engineering has received a fresh
impetus. Engineering proteins for thermostability is a particularly exciting and challenging …

We describe an efficient way to generate combinatorial libraries of stable, soluble and well-
expressed ankyrin repeat (AR) proteins. Using a combination of sequence and structure …

The progress achieved by several groups in the field of computational protein design shows
that successful design methods include two major features: efficient algorithms to deal with …

Previously, we calculated a consensus amino acid sequence from 13 homologous fungal
phytases. A synthetic gene was constructed and recombinantly expressed. Surprisingly …

Nucleotide regulates the affinity of the bacterial chaperonin GroEL for protein substrates.
GroEL binds protein substrates with high affinity in the absence of ATP and with low affinity …

Here, we report a new computational method, called sheetminer, for mining β-sheets in the
density maps at intermediate resolutions of 6 to 10Å. The method employs a multi-step ad …

Many recombinant proteins (rPRTs) have a high bioactivity and some of them may
eventually be classified as drugs beneficial to human health, recombinant human protein …

We report the development of two computational methods to assist density map
interpretation at intermediate resolutions: sheettracer for building pseudo-Cα models of β …

Consensus engineering has been used to increase the stability of a number of different
proteins, either by creating consensus proteins from scratch or by modifying existing proteins …

Background To develop protein therapeutics from exogenous sources, it is necessary to
mitigate the risks of eliciting an anti-biotherapeutic immune response. A key aspect of the …