Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …

Sortase‐mediated ligation (SML) has emerged as a powerful and versatile methodology for
site‐specific protein conjugation, functionalization/labeling, immobilization, and design of …

The transpeptidase sortase A of Staphylococcus aureus (Sa‐SrtA) is a valuable tool in
protein chemistry. The native enzyme anchors surface proteins containing a highly …

Sortases cleave short peptide motif sequences at the C-terminal end of secreted surface
protein precursors and either attach these polypeptides to the peptidoglycan of Gram …

Highlights•Sortase covalently links surface proteins and pili with the CWSS to
peptidoglycan.•Unfolded precursors of surface proteins and pili are folded non oxidatively or …

Mesenchymal stem cells are the focus of intense research in bone development and
regeneration. The potential of microparticles as modulating moieties of osteogenic response …

Adhesive pili in Gram-positive bacteria represent a variety of extracellular multiprotein
polymers that mediate bacterial colonization of specific host tissues and associated …

Proteins that are site-specifically modified with peptides and chemicals can be used as
novel therapeutics, imaging tools, diagnostic reagents and materials. However, there are …

Streptococcus oralis is a member of the mitis group of oral streptococci and an early
colonizer in dental plaque biofilm, a major cause of periodontal disease, dental caries, and …

Sortase enzymes are attractive antivirulence drug targets that attach virulence factors to the
surface of Staphylococcus aureus and other medically significant bacterial pathogens. Prior …