Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
DNA synthesis technologies to close the gene writing gap
A Hoose, R Vellacott, M Storch, PS Freemont… - Nature Reviews …, 2023 - nature.com
Synthetic DNA is of increasing demand across many sectors of research and commercial
activities. Engineering biology, therapy, data storage and nanotechnology are set for rapid …
activities. Engineering biology, therapy, data storage and nanotechnology are set for rapid …
Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system
Y Jiang, B Chen, C Duan, B Sun, J Yang… - Applied and …, 2015 - Am Soc Microbiol
An efficient genome-scale editing tool is required for construction of industrially useful
microbes. We describe a targeted, continual multigene editing strategy that was applied to …
microbes. We describe a targeted, continual multigene editing strategy that was applied to …
Fourth generation biofuel from genetically modified algal biomass: Challenges and future directions
Genetic engineering applications in the field of biofuel are rapidly expanding due to their
potential to boost biomass productivity while lowering its cost and enhancing its quality …
potential to boost biomass productivity while lowering its cost and enhancing its quality …
Synthetic biology to access and expand nature's chemical diversity
Bacterial genomes encode the biosynthetic potential to produce hundreds of thousands of
complex molecules with diverse applications, from medicine to agriculture and materials …
complex molecules with diverse applications, from medicine to agriculture and materials …
Systems strategies for developing industrial microbial strains
Industrial strain development requires system-wide engineering and optimization of cellular
metabolism while considering industrially relevant fermentation and recovery processes. It …
metabolism while considering industrially relevant fermentation and recovery processes. It …
Synthetic DNA synthesis and assembly: putting the synthetic in synthetic biology
RA Hughes, AD Ellington - Cold Spring Harbor …, 2017 - cshperspectives.cshlp.org
The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes,
circuits, and even entire genomes by gene synthesis methods has become an enabling …
circuits, and even entire genomes by gene synthesis methods has become an enabling …
Large-scale de novo DNA synthesis: technologies and applications
For over 60 years, the synthetic production of new DNA sequences has helped researchers
understand and engineer biology. Here we summarize methods and caveats for the de novo …
understand and engineer biology. Here we summarize methods and caveats for the de novo …
Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering
Improvements in DNA synthesis and sequencing have underpinned comprehensive
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …
Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
Designing an optimal microbial cell factory often requires overexpression, knock-down, and
knock-out of multiple gene targets. Unfortunately, such rewiring of cellular metabolism is …
knock-out of multiple gene targets. Unfortunately, such rewiring of cellular metabolism is …