V-ATPases are membrane-embedded protein complexes that function as ATP hydrolysis-
driven proton pumps. V-ATPases are the primary source of organellar acidification in all …

Lysosomes are the main catabolic organelles of a cell and play a pivotal role in a plethora of
cellular processes, including responses to nutrient availability and composition, stress …

Ascertaining the impact of uncharacterized perturbations on the cell is a fundamental
problem in biology. Here, we describe how a single assay can be used to monitor hundreds …

Mitochondria and lysosomes are functionally linked, and their interdependent decline is a
hallmark of aging and disease. Despite the long-standing connection between these …

The vacuolar H+-ATPase of the yeast Saccharomyces cerevisiae is composed of a complex
of peripheral subunits (the V 1 sector) attached to an integral membrane complex (the V 0 …

F1Fo ATP synthases produce most of the ATP in the cell. F-type ATP synthases have been
investigated for more than 50 years, but a full understanding of their molecular mechanisms …

All eukaryotic cells contain multiple acidic organelles, and V-ATPases are central players in
organelle acidification. Not only is the structure of V-ATPases highly conserved among …

The PMC1 gene in Saccharomyces cerevisiae encodes a vacuolar Ca2+ ATPase required
for growth in high-Ca2+ conditions. Previous work showed that Ca2+ tolerance can be …

Ca2+ ATPases deplete the cytosol of Ca2+ ions and are crucial to cellular Ca2+
homeostasis. The PMC1 gene of Saccharomyces cerevisiae encodes a vacuole membrane …

The vacuolar H+-ATPase (V-ATPase) is one of the most fundamental enzymes in nature. It
functions in almost every eukaryotic cell and energizes a wide variety of organelles and …