Hydrolase enzymes, including proteases, are encoded by 2–3% of the genes in the human
genome and 14% of these enzymes are active drug targets. However, the activities and …

Proximity biotinylation based on Escherichia coli BirA enzymes such as BioID (BirA*) and
TurboID is a key technology for identifying proteins that interact with a target protein in a cell …

Covalent catalytic intermediates provide valuable information for revealing the catalytic
mechanism, probing the enzyme activity and identifying substrate specificity. However …

The toll-like receptor 4 (TLR4) is a central regulator of innate immunity that primarily
recognizes bacterial lipopolysaccharide cell wall constituents to trigger cytokine secretion …

The endoplasmic reticulum (ER)-resident intramembrane rhomboid protease RHBDL4
generates metastable protein fragments and together with the ER-associated degradation …

The amyloid precursor protein (APP) is a key protein in Alzheimer's disease synthesized in
the endoplasmic reticulum (ER) and translocated to the plasma membrane where it …

Phosphorylation modifications play important regulatory roles in most biological processes.
However, the functional assignment for the vast majority of the identified phosphosites …

The physiological functions of the rhomboid-related protein 4 (RHBDL4) are emerging, but
their molecular details remain unclear. Because increased expression of RHBDL4 has been …

Mitochondria are fundamental for life and require balanced ion exchange to maintain proper
functioning. The mitochondrial cation exchanger LETM1 sparks interest because of its …

In metazoans, the architecture of the endoplasmic reticulum (ER) differs between cell types
and undergoes major changes throughout the cell cycle and according to physiological …