Isoelectric focusing in immobilized pH gradients: principle, methodology and some applications

B Bjellqvist, K Ek, PG Righetti, E Gianazza… - Journal of biochemical …, 1982 - Elsevier
A new technique for generating pH gradients in isoelectric focusing is described, based on
the principle that the buffering groups are covalently linked to the matrix used as …

Visualization of the binding, endocytosis, and transcytosis of low-density lipoprotein in the arterial endothelium in situ.

E Vasile, M Simionescu, N Simionescu - The Journal of cell biology, 1983 - rupress.org
We investigated the interaction and transport of low-density lipoprotein (LDL) through the
arterial endothelium in rat aorta and coronary artery, by perfusing in situ native, untagged …

Growth hormone and the adipose conversion of 3T3 cells

M Morikawa, T Nixon, H Green - Cell, 1982 - cell.com
Cultured preadipose 3T3 cells are induced or enabled to undergo adipose conversion in the
presence of an extract of pituitary gland. Adipogenic activity is found in standard growth …

Microsomal glutathione transferase: purification in unactivated form and further characterization of the activation process, substrate specificity and amino acid …

R Morgenstern, JW DePIERRE - European journal of …, 1983 - Wiley Online Library
The procedure developed for purification of the N‐ethylmaleimide‐activated microsomal
glutathione transferase was applied successfully to isolation of this same enzyme in …

Histamine receptors of the microvascular endothelium revealed in situ with a histamine-ferritin conjugate: characteristic high-affinity binding sites in venules.

C Heltianu, M Simionescu, N Simionescu - The Journal of cell biology, 1982 - rupress.org
Histamine covalently bound to glutaraldehyde-activated ferritin was prepared as either
monomers or as small aggregates of approximately 0.05 to 0.15 micrometer Diam, suitable …

Kytococcus sedentarius, the organism associated with pitted keratolysis, produces two keratin‐degrading enzymes

CM Longshaw, JD Wright, AM Farrell… - Journal of applied …, 2002 - academic.oup.com
Aims: To determine characteristics of the extracellular enzyme activity of Kytococcus
sedentarius on human callus. Methods and Results: A concentrate of a continuous culture …

Differential induction of cytosolic eposide hydrolase, microsomal epoxide hydrolase, and glutathione S-transferase activities

BD Hammock, K Ota - Toxicology and applied pharmacology, 1983 - Elsevier
Three major enzyme systems have been shown to metabolize epozidized xenobiotics in
vertebrate tissues, and this study demonstrates that these enzyme systems can be …

Comparative studies on the protein composition of hymenopteran venom reservoirs

J Leluk, J Schmidt, D Jones - Toxicon, 1989 - Elsevier
Abstract J. Leluk, J. Schmidt and D. Jones. Comparative studies on the protein composition
of hymenopteran venom reservoirs. Toxicon27, 105–114, 1989.—The proteins of venom …

Pirimicarb resistance in the melon and cotton aphid, Aphis gossypii Glover

C Furk, DF Powell, S Heyd - Plant Pathology, 1980 - Wiley Online Library
The resistance to pirimicarb of populations of Aphis gossypii Glover from several
glasshouses in England was studied in the laboratory by topical applications of insecticide …

Preservation of the microbiological and biochemical quality of raw milk by carbon dioxide addition: a pilot-scale study

P Ruas-Madiedo, JC Bada-Gancedo… - Journal of Food …, 1996 - Elsevier
Carbon dioxide treatment of refrigerated raw milk was evaluated as a method for extending
storage life by inhibiting growth of psychrotrophic bacteria and other bacterial groups in raw …