Technological advances in mammalian systems are providing new tools to identify the
molecular components of signalling pathways. Foremost among these tools is the ability to …

RNA interference (RNAi) is an evolutionarily conserved defence mechanism whereby genes
are specifically silenced through degradation of messenger RNAs; this process is mediated …

For decades, studying the behavioral effects of individual drugs and genetic mutations has
been at the heart of efforts to understand and treat nervous system disorders. High …

In mammals, the fetal liver is the first site of definitive erythropoiesis—the generation of
mature, enucleated red cells. The functional unit for definitive erythropoiesis is the …

Olfactory sensory neurons expose to the inhaled air chemosensory cilia which bind odorants
and operate as transduction organelles. Odorant receptors in the ciliary membrane activate …

Germinal vesicle (GV) breakdown is fundamental for maturation of fully grown,
developmentally competent, mammalian oocytes. Bidirectional communication between …

The cilia of mammalian olfactory receptor neurons (ORNs) represent the sensory interface
that is exposed to the air within the nasal cavity. The cilia are the site where odorants bind to …

Differential detergent fractionation (DDF), which relies on detergents to sequentially extract
proteins from eukaryotic cells, has been used to increase proteome coverage of 2D-PAGE …

Zebrafish larvae are particularly amenable to whole animal small molecule screens1, 2 due
to their small size and relative ease of manipulation and observation, as well as the fact that …

Here we describe the establishment of microscope-based functional screening assays in
intact cells that allow us to systematically identify new proteins involved in secretory …