Many replication proteins assemble on the pre-RC-formed replication origins and constitute
the pre-initiation complex (pre-IC). This complex formation facilitates the conversion of Mcm2 …

Eukaryotic cells must accurately and efficiently duplicate their genomes during each round
of the cell cycle. Multiple linear chromosomes, an abundance of regulatory elements, and …

Genetic studies with S. cerevisiae Polδ (pol3-L612M) and Polε (pol2-M644G) mutant alleles,
each of which display a higher rate for the generation of a specific mismatch, have led to the …

Three eukaryotic DNA polymerases are essential for genome replication. Polymerase (Pol)
α–primase initiates each synthesis event and is rapidly replaced by processive DNA …

Eukaryotic origin firing depends on assembly of the Cdc45-MCM-GINS (CMG) helicase. A
key step is the recruitment of GINS that requires the leading-strand polymerase Pol epsilon …

The eukaryotic leading strand DNA polymerase (Pol) ε contains 4 subunits, Pol2, Dpb2,
Dpb3 and Dpb4. Pol2 is a fusion of two B-family Pols; the N-terminal Pol module is catalytic …

Background The eukaryotic replisome is a critical determinant of genome integrity with a
complex structure that remains poorly characterized. A central unresolved issue is how the …

DNA polymerase ε (POLE) is a four-subunit complex and the major leading strand
polymerase in eukaryotes. Budding yeast orthologs of POLE3 and POLE4 promote Polε …

In eukaryotes three DNA polymerases (Pols), α, δ, and ε, are tasked with bulk DNA synthesis
of nascent strands during genome duplication. Most evidence supports a model where Pol α …

Abstract DNA Polymerase Epsilon (Pol ε) is one of three DNA Polymerases (along with Pol δ
and Pol α) required for nuclear DNA replication in eukaryotes. Pol ε is comprised of four …