This paper aims to discuss various aspects of the use of reference genes in qPCR technique
used in the thousands of present studies. Most frequently, these are housekeeping genes …

Given the highly dynamic nature of mRNA transcription and the potential variables
introduced in sample handling and in the downstream processing steps (Garson et …

Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …

Plant adaptive responses to drought are coordinated by adjusting growth and
developmental processes as well as molecular and cellular activities. The root system is the …

Background Usually the reference genes used in gene expression analysis have been
chosen for their known or suspected housekeeping roles, however the variation observed in …

The development of shoot-borne roots, or adventitious roots, is indispensable for mass
propagation of elite genotypes. It is a complex genetic trait with a high phenotypic plasticity …

Nicotiana benthamiana is the most widely-used experimental host in plant virology. The
recent release of the draft genome sequence for N. benthamiana consolidates its role as a …

Background RT-qPCR is a preferred method for rapid and reliable quantification of gene
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …

Leaf aging is a highly regulated developmental process, which is also influenced profoundly
by diverse environmental conditions. Accumulating evidence in recent years supports that …

Quantitative RT-PCR (reverse transcription polymerase chain reaction, also known as qRT-
PCR or real-time RT-PCR) has been used in large proportions of transcriptome analyses …