Applying a high voltage to a liquid passing through a capillary disintegrates the liquid
meniscus to generate a fine spray of charged droplets. As the droplets evaporate, polar …

Small molecule drug discovery has been propelled by the continual development of novel
scientific methodologies to occasion therapeutic advances. Although established …

Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …

Biological membranes formed of amphipathic lipid bilayers provide chemical barriers to
separate cells from their external environments and define subcellular compartments …

Here we describe a detailed protocol for both data collection and interpretation with respect
to ion mobility–mass spectrometry analysis of large protein assemblies. Ion mobility is a …

Nucleic acids have been among the first targets for antitumor drugs and antibiotics. With the
unveiling of new biological roles in regulation of gene expression, specific DNA and RNA …

Abstract I. Introduction 00 II. Electrospray Ionization of Biomacromolecules 00 III. Mass
Analyzers for Biomacromolecular Mass Spectrometry 00 A. Collisional Cooling and/or …

Mass spectrometry-based methods have become increasingly important in structural biology—
in particular for large and dynamic, even heterogeneous assemblies of biomolecules. Native …

Small heat shock proteins (sHSPs) associate with nuclei, cytoskeleton and membranes, and
as molecular chaperones they bind partially denatured proteins, thereby preventing …

Proteins carry out the most difficult tasks in living cells. They do so by interacting specifically
with other molecules. This requires that they fold to a unique, globular conformation that is …