CRISPR screening in hematology research: from bulk to single-cell level
The CRISPR genome editing technology has revolutionized the way gene function is
studied. Genome editing can be achieved in single genes or for thousands of genes …
studied. Genome editing can be achieved in single genes or for thousands of genes …
Multiplexing methods for simultaneous large‐scale transcriptomic profiling of samples at single‐cell resolution
Barcoding technology has greatly improved the throughput of cells and genes detected in
single‐cell RNA sequencing (scRNA‐seq) studies. Recently, increasing studies have paid …
single‐cell RNA sequencing (scRNA‐seq) studies. Recently, increasing studies have paid …
Targeted Perturb-seq enables genome-scale genetic screens in single cells
D Schraivogel, AR Gschwind, JH Milbank, DR Leonce… - Nature …, 2020 - nature.com
The transcriptome contains rich information on molecular, cellular and organismal
phenotypes. However, experimental and statistical limitations constrain sensitivity and …
phenotypes. However, experimental and statistical limitations constrain sensitivity and …
Prime-seq, efficient and powerful bulk RNA sequencing
Cost-efficient library generation by early barcoding has been central in propelling single-cell
RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA …
RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA …
Sample-multiplexing approaches for single-cell sequencing
Single-cell sequencing is widely used in biological and medical studies. However, its
application with multiple samples is hindered by inefficient sample processing, high …
application with multiple samples is hindered by inefficient sample processing, high …
New approach methodologies to facilitate and improve the hazard assessment of non-genotoxic carcinogens—a PARC project
Carcinogenic chemicals, or their metabolites, can be classified as genotoxic or non-
genotoxic carcinogens (NGTxCs). Genotoxic compounds induce DNA damage, which can …
genotoxic carcinogens (NGTxCs). Genotoxic compounds induce DNA damage, which can …
HyPR-seq: single-cell quantification of chosen RNAs via hybridization and sequencing of DNA probes
JL Marshall, BR Doughty… - Proceedings of the …, 2020 - National Acad Sciences
Single-cell quantification of RNAs is important for understanding cellular heterogeneity and
gene regulation, yet current approaches suffer from low sensitivity for individual transcripts …
gene regulation, yet current approaches suffer from low sensitivity for individual transcripts …
scPNMF: sparse gene encoding of single cells to facilitate gene selection for targeted gene profiling
Motivation Single-cell RNA sequencing (scRNA-seq) captures whole transcriptome
information of individual cells. While scRNA-seq measures thousands of genes, researchers …
information of individual cells. While scRNA-seq measures thousands of genes, researchers …
Multiparametric assays for accelerating early drug discovery
Drug discovery campaigns are hampered by substantial attrition rates largely due to a lack
of efficacy and safety reasons associated with candidate drugs. This is true in particular for …
of efficacy and safety reasons associated with candidate drugs. This is true in particular for …
Potential of genomic selection and integrating “omics” data for disease evaluation in wheat
Diseases are among the most important limiting factors for wheat production. Breeding for
fungal diseases of wheat, primarily for rusts and Fusarium head blight (FHB), are major …
fungal diseases of wheat, primarily for rusts and Fusarium head blight (FHB), are major …