Spin-label electron paramagnetic resonance (EPR) spectroscopy is a versatile molecular probe method that finds wide application in molecular biophysics and structural biology. This …
Molecular oxygen (O2) is the perfect probe molecule for membrane studies carried out using the saturation recovery EPR technique. O2 is a small, paramagnetic, hydrophobic enough …
L Mainali, M Raguz, WJ O'Brien, WK Subczynski - Biophysical Journal, 2013 - cell.com
The organization and physical properties of lens lipid membranes made from the total lipids extracted from the human lens cortex and nucleus of 41-to 60-year-old donors were …
L Mainali, M Raguz, WJ O'Brien… - European Biophysics …, 2015 - Springer
Human lens-lipid membranes prepared from the total lipids extracted from clear and cataractous lens cortexes and nuclei of 61–70-year-old donors by use of a rapid solvent …
R Timsina, S Wellisch, D Haemmerle… - International Journal of …, 2022 - mdpi.com
Several studies reported that α-crystallin concentrations in the eye lens cytoplasm decrease with a corresponding increase in membrane-bound α-crystallin with age and cataracts. The …
L Mainali, WJ O'Brien, R Timsina - Current eye research, 2021 - Taylor & Francis
ABSTRACT Purpose/Aim: The amount of membrane-bound α-crystallin increases significantly with age and cataract formation, accompanied by a corresponding decline in …
L Mainali, JS Hyde, WK Subczynski - Journal of Magnetic Resonance, 2013 - Elsevier
Conventional and saturation-recovery (SR) EPR at W-band (94GHz) using phosphatidylcholine spin labels (labeled at the alkyl chain [n-PC] and headgroup [T-PC]) to …
L Mainali, M Raguz, WK Subczynski - European biophysics journal, 2012 - Springer
EPR spin-labeling methods were used to investigate the order and fluidity of alkyl chains, the hydrophobicity of the membrane interior, and the order and motion of cholesterol …
M Raguz, L Mainali, WJ O'Brien… - Experimental eye …, 2015 - Elsevier
The results reported here clearly document changes in the properties and the organization of fiber-cell membrane lipids that occur with age, based on electron paramagnetic …