Functions of cholesterol and the cholesterol bilayer domain specific to the fiber-cell plasma membrane of the eye lens

WK Subczynski, M Raguz, J Widomska… - The Journal of …, 2012 - Springer
The most unique feature of the eye lens fiber-cell plasma membrane is its extremely high
cholesterol content. Cholesterol saturates the bulk phospholipid bilayer and induces …

[图书][B] Spin-label electron paramagnetic resonance spectroscopy

D Marsh - 2019 - taylorfrancis.com
Spin-label electron paramagnetic resonance (EPR) spectroscopy is a versatile molecular
probe method that finds wide application in molecular biophysics and structural biology. This …

Molecular oxygen as a probe molecule in EPR spin labeling studies of membrane structure and dynamics

WK Subczynski, J Widomska, M Raguz… - Oxygen, 2022 - mdpi.com
Molecular oxygen (O2) is the perfect probe molecule for membrane studies carried out using
the saturation recovery EPR technique. O2 is a small, paramagnetic, hydrophobic enough …

Properties of membranes derived from the total lipids extracted from the human lens cortex and nucleus

L Mainali, M Raguz, WJ O'Brien, WK Subczynski - Biophysical Journal, 2013 - cell.com
The organization and physical properties of lens lipid membranes made from the total lipids
extracted from the human lens cortex and nucleus of 41-to 60-year-old donors were …

Properties of membranes derived from the total lipids extracted from clear and cataractous lenses of 61–70-year-old human donors

L Mainali, M Raguz, WJ O'Brien… - European Biophysics …, 2015 - Springer
Human lens-lipid membranes prepared from the total lipids extracted from clear and
cataractous lens cortexes and nuclei of 61–70-year-old donors by use of a rapid solvent …

Binding of Alpha-Crystallin to Cortical and Nuclear Lens Lipid Membranes Derived from a Single Lens

R Timsina, S Wellisch, D Haemmerle… - International Journal of …, 2022 - mdpi.com
Several studies reported that α-crystallin concentrations in the eye lens cytoplasm decrease
with a corresponding increase in membrane-bound α-crystallin with age and cataracts. The …

Interaction of alpha-crystallin with phospholipid membranes

L Mainali, WJ O'Brien, R Timsina - Current eye research, 2021 - Taylor & Francis
ABSTRACT Purpose/Aim: The amount of membrane-bound α-crystallin increases
significantly with age and cataract formation, accompanied by a corresponding decline in …

Using spin-label W-band EPR to study membrane fluidity profiles in samples of small volume

L Mainali, JS Hyde, WK Subczynski - Journal of Magnetic Resonance, 2013 - Elsevier
Conventional and saturation-recovery (SR) EPR at W-band (94GHz) using
phosphatidylcholine spin labels (labeled at the alkyl chain [n-PC] and headgroup [T-PC]) to …

Phases and domains in sphingomyelin–cholesterol membranes: structure and properties using EPR spin-labeling methods

L Mainali, M Raguz, WK Subczynski - European biophysics journal, 2012 - Springer
EPR spin-labeling methods were used to investigate the order and fluidity of alkyl chains,
the hydrophobicity of the membrane interior, and the order and motion of cholesterol …

Lipid domains in intact fiber-cell plasma membranes isolated from cortical and nuclear regions of human eye lenses of donors from different age groups

M Raguz, L Mainali, WJ O'Brien… - Experimental eye …, 2015 - Elsevier
The results reported here clearly document changes in the properties and the organization
of fiber-cell membrane lipids that occur with age, based on electron paramagnetic …