In the ten years since the discovery of the first anti-CRISPR (Acr) proteins, the number of
validated Acrs has expanded rapidly, as has our understanding of the diverse mechanisms …

Comprehensive Summary The control of protein functions with light is valuable for
spatiotemporal probing of biological systems. Current small‐molecule photo‐modulation …

Rapid advancements in biotechnology have enabled the development of new therapeutic
modalities for refractory diseases. Among these, stem cell-derived β (SC-β) cells are …

CRISPR-Cas9 system has emerged as an effective tool for sequence-specific gene
knockout through non-homologous end joining (NHEJ). However, the inefficient precise …

The application of CRISPR-Cas systems to genome editing has revolutionized experimental
biology and is an emerging gene and cell therapy modality. CRISPR-Cas systems target off …

The need for precision control of CRISPR-Cas9 genome editing has created a demand for
anti-CRISPR molecules. Recently, the first class of small-molecule Cas9 inhibitors has been …

Promiscuous editing by CRISPR/Cas systems within the human genome is a major
challenge that must be addressed prior to applying these systems therapeutically. In …

Although engineering transplantable tissues for clinical applications is difficult,
cryopreserving those tissues to create pre-made engineered tissue for emergency …

The genome-editing power of the CRISPR-Cas9 system has propelled the development of
novel therapeutic modalities. However, several barriers remain to the safe use of CRISPR …