Optogenetics ushered in a revolution in how neuroscientists interrogate brain function.
Because of technical limitations, the majority of optogenetic studies have used low spatial …

Over the past two decades, genetically encoded calcium indicators (GECIs) have been used
extensively to report intracellular calcium (Ca 2+) dynamics in order to readout neuronal and …

Land-walking vertebrates maintain a desirable posture by finely controlling muscles. It is
unclear whether fish also finely control posture in the water. Here, we showed that larval …

Two-photon, single-cell resolution optogenetics based on holographic light-targeting
approaches enables the generation of precise spatiotemporal neuronal activity patterns and …

Optical recordings of neuronal activity at cellular resolution represent an invaluable tool to
investigate brain mechanisms. Zebrafish larvae is one of the few model organisms where …

We developed a multi-unit microscope for all-optical inter-layers circuits interrogation. The
system performs two-photon (2P) functional imaging and 2P multiplexed holographic …

The development of efficient genetically encoded indicators and actuators has opened up
the possibility of reading and manipulating neuronal activity in living tissues with light. To …

Monosynaptic connectivity mapping is crucial for building circuit-level models of neural
computation. Two-photon optogenetic stimulation, when combined with whole-cell …

Significance Two-photon optogenetics combines nonlinear excitation with noninvasive
activation of neurons to enable the manipulation of neural circuits with a high degree of …

Light-sheet microscopy is a powerful method for imaging small translucent samples in vivo,
owing to its unique combination of fast imaging speeds, large field of view, and low …