Enzymes are considered as functional proteins, also known as biocatalysts, which are
required for normal cellular function. The commercial production of enzymes with distinct …

Many enzymes are efficiently produced by microbes. However, the use of natural enzymes
as biocatalysts has limitations such as low catalytic efficiency, low activity, and low stability …

Fusion constructs are used to improve the properties of or impart novel functionality to
proteins for biotechnological applications. The biochemical characteristics of enzymes or …

This study aimed to enhance the expression level of a novel trypsin gene from Streptomyces
fradiae ATCC14544 in Komagataella phaffii GS115 through the combinational use of …

A potential myo-inositol oxygenase (ppMIOX) was identified as a functional enzyme and a
glucaric acid synthetic pathway was firstly constructed in Pichia pastoris. Coexpression of …

Pichia pastoris:(reclassified as Komagataella phaffii), a methylotrophic yeast strain has been
widely used for heterologous protein production because of its unique advantages, such as …

Keratinase is an attractive industrial enzyme that can specifically catalyze keratin waste to
obtain value‐added products. A challenge to the application of keratinase is improving …

Streptomyces griseus trypsin (SGT) possesses enzymatic properties similar to mammalian
trypsins and has great potential applications in the leather processing, bioethanol, detergent …

Leech hyaluronidase (LHAase) was recently cloned and successfully expressed in Pichia
pastoris. To increase its secretory expression level, four signal peptides (nsB, YTP1, SCS3 …

Cocoonase, a protein that is produced by the silkworm (Bombyx mori), is thought to
specifically digest the sericin protein of the cocoon and has a high homology with trypsin …