Microtechnology-based methods for organoid models
V Velasco, SA Shariati, R Esfandyarpour - Microsystems & …, 2020 - nature.com
Innovations in biomaterials and stem cell technology have allowed for the emergence of
novel three-dimensional (3D) tissue-like structures known as organoids and spheroids. As a …
novel three-dimensional (3D) tissue-like structures known as organoids and spheroids. As a …
Embryoids, organoids and gastruloids: new approaches to understanding embryogenesis
M Simunovic, AH Brivanlou - Development, 2017 - journals.biologists.com
Cells have an intrinsic ability to self-assemble and self-organize into complex and functional
tissues and organs. By taking advantage of this ability, embryoids, organoids and …
tissues and organs. By taking advantage of this ability, embryoids, organoids and …
Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids
During gastrulation, the pluripotent epiblast self-organizes into the 3 germ layers—
endoderm, mesoderm and ectoderm, which eventually form the entire embryo. Decades of …
endoderm, mesoderm and ectoderm, which eventually form the entire embryo. Decades of …
Opportunities and challenges with stem cell-based embryo models
J Rossant, PPL Tam - Stem Cell Reports, 2021 - cell.com
Stem cell-based embryo models open an unprecedented avenue for modeling
embryogenesis, cell lineage differentiation, tissue morphogenesis, and organogenesis in …
embryogenesis, cell lineage differentiation, tissue morphogenesis, and organogenesis in …
Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells
The creation of human induced pluripotent stem cells (hiPSCs) has provided an
unprecedented opportunity to study tissue morphogenesis and organ development through' …
unprecedented opportunity to study tissue morphogenesis and organ development through' …
lncRNA DIGIT and BRD3 protein form phase-separated condensates to regulate endoderm differentiation
Cooperation between DNA, RNA and protein regulates gene expression and controls
differentiation through interactions that connect regions of nucleic acids and protein domains …
differentiation through interactions that connect regions of nucleic acids and protein domains …
Hydrogel-based bioinks for cell electrowriting of well-organized living structures with micrometer-scale resolution
Bioprinting has become an important tool for fabricating regenerative implants and in vitro
cell culture platforms. However, until today, extrusion-based bioprinting processes are …
cell culture platforms. However, until today, extrusion-based bioprinting processes are …
A stepwise model of reaction-diffusion and positional information governs self-organized human peri-gastrulation-like patterning
How position-dependent cell fate acquisition occurs during embryogenesis is a central
question in developmental biology. To study this process, we developed a defined, high …
question in developmental biology. To study this process, we developed a defined, high …
A wave of WNT signaling balanced by secreted inhibitors controls primitive streak formation in micropattern colonies of human embryonic stem cells
Long-range signaling by morphogens and their inhibitors define embryonic patterning yet
quantitative data and models are rare, especially in humans. Here, we use a human …
quantitative data and models are rare, especially in humans. Here, we use a human …
Membrane potential drives the exit from pluripotency and cell fate commitment via calcium and mTOR
E Sempou, V Kostiuk, J Zhu, M Cecilia Guerra… - Nature …, 2022 - nature.com
Transitioning from pluripotency to differentiated cell fates is fundamental to both embryonic
development and adult tissue homeostasis. Improving our understanding of this transition …
development and adult tissue homeostasis. Improving our understanding of this transition …