Development of a naked eye CRISPR-Cas12a and-Cas13a multiplex point-of-care detection of genetically modified swine
Y Wang, L Fu, D Tao, X Han, B Xu, M Deng… - ACS synthetic …, 2023 - ACS Publications
The Rapid Visual CRISPR (RAVI-CRISPR) assay employs Cas12a and Cas13a enzymes
for precise gene detection in a sample. However, RAVI-CRISPR is limited in single-tube …
for precise gene detection in a sample. However, RAVI-CRISPR is limited in single-tube …
Feasibility of implementing RPA coupled with CRISPR-Cas12a (RPA-Cas12a) for Hepatozoon canis detection in dogs
S Paenkaew, A Poommouang, W Pradit… - Veterinary …, 2024 - Elsevier
Hepatozoonosis, caused by the protozoan Hepatozoon canis, is a prevalent blood disease
affecting owned and stray dogs and cats. The prevalence of these parasites among …
affecting owned and stray dogs and cats. The prevalence of these parasites among …
A newly developed droplet digital PCR for Ehrlichia canis detection: Comparisons to conventional PCR and blood smear techniques
S Wichianchot, N Hongsrichan… - Journal of Veterinary …, 2022 - jstage.jst.go.jp
Canine monocytic ehrlichiosis caused by Ehrlichia canis infection is a life-threatening vector-
borne disease in dogs worldwide. Routine blood smear has very low sensitivity and cannot …
borne disease in dogs worldwide. Routine blood smear has very low sensitivity and cannot …
Multiple blood pathogen infections in apparently healthy sheltered dogs in southern Thailand
In developing countries such as Thailand, free-ranging dogs are frequently involved in road
accidents and contribute to the cost of public healthcare. Shelters play a vital role in …
accidents and contribute to the cost of public healthcare. Shelters play a vital role in …
Novel high-throughput multiplex qPCRs for the detection of canine vector-borne pathogens in the Asia-Pacific
The Asia-Pacific hosts a large diversity of canine vector-borne pathogens (VBPs) with some
of the most common and most pathogenic, generating significant mortality as well as a …
of the most common and most pathogenic, generating significant mortality as well as a …
Haemoprotozoan and haemorickettsial carrier status in pet and community owned dogs of south India
GS Kumar, A Varghese, CAF Bora, PK Hembram… - … : Regional Studies and …, 2024 - Elsevier
The increasing population of dogs and changes in the climatic conditions have resulted in
the emergence and re-emergence of vector-borne diseases in canines. These vectors borne …
the emergence and re-emergence of vector-borne diseases in canines. These vectors borne …
Development and validation of multiplex SYBR Green real-time PCR assays for detection and molecular surveillance of four tick-borne canine haemoparasites
Two multiplex SYBR Green based real-time PCR assays were standardized and evaluated
to detect DNA from four canine haemoparasites (Babesia gibsoni, Babesia vogeli, Ehrlichia …
to detect DNA from four canine haemoparasites (Babesia gibsoni, Babesia vogeli, Ehrlichia …
Duplex real-time PCR methods for molecular detection and characterization of canine tick-borne haemoparasites from Punjab state, India
Background Microscopy is a routinely used technique for the diagnosis of canine tick-borne
haemoparasitic diseases in various clinical laboratories worldwide. In an attempt to provide …
haemoparasitic diseases in various clinical laboratories worldwide. In an attempt to provide …
Molecular Survey of Tick-Borne Haemoparasites of Dogs by Multiplex Polymerase Chain Reaction from Punjab, India
Purpose Tick-transmitted parasites as Babesia gibsoni, Babesia vogeli, Ehrlichia canis, and
Hepatozoon canis are major health concern for dogs. Owing to prevalence and infection …
Hepatozoon canis are major health concern for dogs. Owing to prevalence and infection …
Detection of canine blood parasites by a multiplex PCR
G Sathish, S Subapriya, M Parthiban, S Vairamuthu - 2021 - indianjournals.com
A multiplex PCR which can be useful for diagnosis of common blood parasitic infections of
dogs in single reaction was standardized. Out of 40 blood samples collected from dogs with …
dogs in single reaction was standardized. Out of 40 blood samples collected from dogs with …