Antigenic variation as a strategy to evade the host adaptive immune response has evolved
in divergent pathogens. Antigenic variation involves restricted, and often mutually exclusive …

The common 5'terminal 22-nt sequence in mRNAs essential for gene expression of
nematodes is formed by trans-splicing of spliced leader RNAs. This process is similar to cis …

The 5'ends of trypanosome mRNAs consist of an identical sequence of 35 nucleotides. This
“mini-exon” sequence is derived from the 5'end of a 137 nucleotide RNA (medRNA). The …

While determining the 5′ ends of C. elegans actin mRNAs, we have discovered a 22
nucleotide spliced leader sequence. The leader sequence is found on mRNA from three of …

Results A 100 Nucleotide Fragment of the SL RNA Is Associated with High Molecular Weight
RNA Throughout this manuscript we will consider the 135 nucleotide SL RNA as having two …

Some Ts in nuclear DNA of trypanosomes and Leishmania are hydroxylated and
glucosylated to yield base J (β-D-glucosyl-hydroxymethyluracil). In Leishmania, about 99 …

Pulsed field gradient gel electrophoresis fractionates chromosome-sized DNA molecules
from T. brucei. About 60% of the DNA remains in or close to the gel slot (large DNA). There …

A single capped oligonucleotide is released from Trypanosoma brucei poly (A)+ RNA upon
digestion with RNase T2. This observation supports the hypothesis that all T. brucei mRNAs …

The genomes of kinetoplastids are organized into polycistronic transcription units that are
flanked by a modified DNA base (base J, beta-D-glucosyl-hydroxymethyluracil). Previous …

Many, if not all, mRNAs in T. brucei start with the same sequence of 35 nucleotides,
separately encoded in clustered so-called mini-exon repeats. From these mini-axon repeats …