Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

M Karbalaei, SA Rezaee… - Journal of cellular …, 2020 - Wiley Online Library
One of the most important branches of genetic engineering is the expression of recombinant
proteins using biological expression systems. Nowadays, different expression systems are …

Industrial Production of Proteins with Pichia pastorisKomagataella phaffii

GD Barone, A Emmerstorfer-Augustin, A Biundo… - Biomolecules, 2023 - mdpi.com
Since the mid-1960s, methylotrophic yeast Komagataella phaffii (previously described as
Pichia pastoris) has received increasing scientific attention. The interest for the industrial …

Biosynthesis of High‐Active Hemoproteins by the Efficient Heme‐Supply Pichia Pastoris Chassis

F Yu, X Zhao, J Zhou, W Lu, J Li, J Chen… - Advanced …, 2023 - Wiley Online Library
Microbial synthesis of valuable hemoproteins has become a popular research topic, and
Pichia pastoris is a versatile platform for the industrial production of recombinant proteins …

1Progress, applications, challenges and prospects of protein purification technology

M Du, Z Hou, L Liu, Y Xuan, X Chen, L Fan… - … in Bioengineering and …, 2022 - frontiersin.org
Protein is one of the most important biological macromolecules in life, which plays a vital
role in cell growth, development, movement, heredity, reproduction and other life activities …

Engineering multifunctional enzymes for agro-biomass utilization

A Sharma, S Balda, N Capalash, P Sharma - Bioresource Technology, 2022 - Elsevier
Lignocellulosic biomass is a plentiful renewable resource that can be converted into a wide
range of high-value-added industrial products. However, the complexity of its structural …

Escherichia coli and Pichia pastoris: microbial cell-factory platform for full-length IgG production

S Krishna, ST Jung, EY Lee - Critical Reviews in Biotechnology, 2024 - Taylor & Francis
Owing to the unmet demand, the pharmaceutical industry is investigating an alternative host
to mammalian cells to produce antibodies for a variety of therapeutic and research …

Recombinant monoclonal antibody production in yeasts: Challenges and considerations

PK Das, A Sahoo, VD Veeranki - International Journal of Biological …, 2024 - Elsevier
Monoclonal antibodies (mAbs) are laboratory-based engineered protein molecules with a
monovalent affinity or multivalent avidity towards a specific target or antigen, which can …

[HTML][HTML] AOX1 promoter-driven expression of yeast-enhanced green fluorescent protein in Pichia pastoris

CS Dharmarathna, S Kumar, YINS Gunawardena… - The Microbe, 2023 - Elsevier
Green fluorescent protein (GFP) is a widely employed reporter for studying various cellular
functions in genetic engineering. Methylotrophic yeast; Pichia pastoris is an excellent host …

[HTML][HTML] 重组rHSA-hFGF21 融合蛋白在毕赤酵母中的表达纯化及活性分析

黄甜甜, 齐剑英, 杨刚刚, 叶贤龙 - 生物工程学报, 2022 - cjb.ijournals.cn
人成纤维细胞生长因子21 (human fibroblast growth factor 21, hFGF21) 已成为改善血糖和脂质
代谢的候选药物, 但却存在半衰期短和易被体内代谢等缺点, 导致靶组织利用率低 …

Cloning and high-level expression of β-xylosidase from Selenomonas ruminantium in Pichia pastoris by optimizing of pH, methanol concentration and temperature …

E Dehnavi, SOR Siadat, MF Roudsari… - Protein Expression and …, 2016 - Elsevier
Abstract β-xylosidase and several other glycoside hydrolase family members, including
xylanase, cooperate together to degrade hemicelluloses, a commonly found xylan polymer …