The field of synthetic biology is evolving at a fast pace. It is advancing beyond single‐gene
alterations in single hosts to the logical design of complex circuits and the development of …

Synthetic genetic circuits offer the potential to wield computational control over biology, but
their complexity is limited by the accuracy of mathematical models. Here, we present …

Cell‐free expression systems enable rapid prototyping of genetic programs in vitro.
However, current throughput of cell‐free measurements is limited by the use of channel …

Abstract Catalytically dead Cas9 (dCas9) is a programmable transcription factor that can be
targeted to promoters through the design of small guide RNAs (sgRNAs), where it can …

Background Corynebacterium glutamicum is an important industrial microorganism used for
the production of many valuable compounds, especially amino acids and their derivatives …

Transcriptional programming leverages systems of engineered transcription factors to impart
decision-making (eg, Boolean logic) in chassis cells. The number of components used to …

Biological computing is a promising field with potential applications in biosafety,
environmental monitoring, and personalized medicine. Here we present work on the design …

Bacterial promoters are usually formed by multiple cis-regulatory elements recognized by a
plethora of transcriptional factors (TFs). From those, global regulators are key elements …

Protein-based switches that respond to different inputs to regulate cellular outputs, such as
gene expression, are central to synthetic biology. For increased controllability, multi-input …

RNA-based regulators are promising tools for building synthetic biological systems that
provide a powerful platform for achieving a complex regulation of transcription and …