Cell-free biosensors for rapid detection of water contaminants

JK Jung, KK Alam, MS Verosloff, DA Capdevila… - Nature …, 2020 - nature.com
Lack of access to safe drinking water is a global problem, and methods to reliably and easily
detect contaminants could be transformative. We report the development of a cell-free in …

Isothermal nucleic acid amplification technology for rapid detection of virus

Z Wei, X Wang, H Feng, F Ji, D Bai… - Critical Reviews in …, 2023 - Taylor & Francis
While the research field and industrial market of in vitro diagnosis (IVD) thrived during and
post the COVID-19 pandemic, the development of isothermal nucleic acid amplification test …

Contemporary tools for regulating gene expression in bacteria

R Kent, N Dixon - Trends in Biotechnology, 2020 - cell.com
Insights from novel mechanistic paradigms in gene expression control have led to the
development of new gene expression systems for bioproduction, control, and sensing …

Dissipative Control over the Toehold‐Mediated DNA Strand Displacement Reaction

E Del Grosso, P Irmisch, S Gentile… - Angewandte Chemie …, 2022 - Wiley Online Library
Here we show a general approach to achieve dissipative control over toehold‐mediated
strand‐displacement, the most widely employed reaction in the field of DNA …

Dissipative operation of pH-responsive DNA-based nanodevices

D Mariottini, D Del Giudice, G Ercolani… - Chemical …, 2021 - pubs.rsc.org
We demonstrate here the use of 2-(4-chlorophenyl)-2-cyanopropanoic acid (CPA) and
nitroacetic acid (NAA) as convenient chemical fuels to drive the dissipative operation of DNA …

Enzyme-driven assembly and disassembly of hybrid DNA–RNA nanotubes

S Agarwal, E Franco - Journal of the American Chemical Society, 2019 - ACS Publications
Living cells have the ability to control the dynamics of responsive assemblies such as the
cytoskeleton by temporally activating and deactivating inert precursors. While DNA …

Evaluating DFHBI-Responsive RNA Light-Up Aptamers as Fluorescent Reporters for Gene Expression

A Climent-Catala, I Casas-Rodrigo, S Iyer… - ACS Synthetic …, 2023 - ACS Publications
Protein-based fluorescent reporters have been widely used to characterize and localize
biological processes in living cells. However, these reporters may have certain drawbacks …

Controlling matter at the molecular scale with DNA circuits

D Scalise, R Schulman - Annual review of biomedical …, 2019 - annualreviews.org
In recent years, a diverse set of mechanisms have been developed that allow DNA strands
with specific sequences to sense information in their environment and to control material …

Allosteric Regulation of Aptamer Affinity through Mechano‐Chemical Coupling

H Qu, M Zheng, Q Ma, L Wang, Y Mao… - Angewandte …, 2023 - Wiley Online Library
The capacity to precisely modulate aptamer affinity is important for a wide variety of
applications. However, most such engineering strategies entail laborious trial‐and‐error …

The cell-free system: A new apparatus for affordable, sensitive, and portable healthcare

CE Copeland, A Langlois, J Kim, YC Kwon - Biochemical Engineering …, 2021 - Elsevier
The cell-free system is a tool that harnesses the complex biological processes outside of
living cells. Owing to the system's superior flexibility and manipulability, it has matured its …