Induced genetic variation in crop plants by random or targeted mutagenesis: convergence and differences

IB Holme, PL Gregersen… - Frontiers in Plant …, 2019 - frontiersin.org
New Breeding Techniques (NBTs) include several new technologies for introduction of new
variation into crop plants for plant breeding, in particular the methods that aim to make …

The regulatory status of genome‐edited crops

JD Wolt, K Wang, B Yang - Plant biotechnology journal, 2016 - Wiley Online Library
Genome editing with engineered nucleases (GEEN) represents a highly specific and
efficient tool for crop improvement with the potential to rapidly generate useful novel …

Precise plant breeding using new genome editing techniques: opportunities, safety and regulation in the EU

F Hartung, J Schiemann - The Plant Journal, 2014 - Wiley Online Library
Several new plant breeding techniques (NPBT s) have been developed during the last
decade, and make it possible to precisely perform genome modifications in plants. The …

New biotechnological tools for the genetic improvement of major woody fruit species

C Limera, S Sabbadini, JB Sweet… - Frontiers in plant …, 2017 - frontiersin.org
The improvement of woody fruit species by traditional plant breeding techniques has several
limitations mainly caused by their high degree of heterozygosity, the length of their juvenile …

Oligonucleotide‐directed mutagenesis for precision gene editing

NJ Sauer, J Mozoruk, RB Miller… - Plant biotechnology …, 2016 - Wiley Online Library
Differences in gene sequences, many of which are single nucleotide polymorphisms,
underlie some of the most important traits in plants. With humanity facing significant …

Biotechnological advances to improve abiotic stress tolerance in crops

MA Villalobos-López, A Arroyo-Becerra… - International Journal of …, 2022 - mdpi.com
The major challenges that agriculture is facing in the twenty-first century are increasing
droughts, water scarcity, flooding, poorer soils, and extreme temperatures due to climate …

From classical mutagenesis to nuclease‐based breeding–directing natural DNA repair for a natural end‐product

M Pacher, H Puchta - The Plant Journal, 2017 - Wiley Online Library
Production of mutants of crop plants by the use of chemical or physical genotoxins has a
long tradition. These factors induce the natural DNA repair machinery to repair damage in …

Genome editing of plants

DD Songstad, JF Petolino, DF Voytas… - Critical Reviews in …, 2017 - Taylor & Francis
Genome editing in organisms via random mutagenesis is a naturally occurring
phenomenon. As a technology, genome editing has evolved from the use of chemical and …

Engineering nucleases for gene targeting: safety and regulatory considerations

K Pauwels, N Podevin, D Breyer, D Carroll, P Herman - New biotechnology, 2014 - Elsevier
Highlights•We review the possibilities and perspectives of nucleases for genetic
engineering.•DNA-recognition, off-target effects are key elements for improvement.•Safety …

Modes of therapeutic delivery in synthetic microbiology

LM Alexander, JP van Pijkeren - Trends in microbiology, 2023 - cell.com
For decades, bacteria have been exploited as vectors for vaccines and therapeutics.
However, the bacterial arsenal used has historically been limited to a few strains …