Considerable advances in media development for the culture of preimplantation mammalian
embryos have been made since mouse embryos were first cultured and successfully …

Amino acids are essential components of media utilized to culture fertilized human eggs to
the blastocyst stage in vitro. Use of such media has led to a significant increase in the …

The culture of murine oocytes and preimplantation embryos in vitro has been used
successfully for many years. However, this practice can result in cellular stress and reduced …

Three-dimensional (3D) bioprinting is a technology to print materials (bioink) with cells into
customized tissues for regeneration or organoids for drug screening applications. Herein, a …

The addition of amino acids to a modified simplex optimized medium (mKSOM) did not
increase the percentage of blastocysts that develop from CF1 mouse ova fertilized in vitro. In …

Mammalian oocytes can reprogram differentiated somatic cells into a totipotent state through
somatic cell nuclear transfer (SCNT), which is known as cloning. Although many mammalian …

BACKGROUND Preimplantation embryos are particularly susceptible to in vitro
developmental blocks. These could be alleviated by lowering culture medium osmolarity …

Cells subjected to sustained high osmolarity almost universally respond by accumulating
compatible organic osmolytes that, in contrast to inorganic ions, are not deleterious even at …

Cloning by somatic cell nuclear transfer is critically dependent upon early events that occur
immediately after nuclear transfer, and possibly additional events that occur in the cleaving …

STUDY QUESTION Which lab-related factors impact the culture system's capacity to
maintain a stable osmolality during human embryo culture? SUMMARY ANSWER Incubator …